Detailed information of TA system TA040826

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 531883..532720
• Replicon: chromosome
• Accession: NZ_AP025754
• Organism: Escherichia sp. KS167_9B strain KS-P079

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: QUE34_RS02600
• Protein ID: WP_105268434.1
• Coordinates: 531883..532425 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: QUE34_RS02605
• Protein ID: WP_001353405.1
• Coordinates: 532409..532720 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE34_RS02580 (527413)	527413..528324	-	912	WP_000705864.1	2-dehydropantoate 2-reductase	-
QUE34_RS02585 (528492)	528492..528983	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
QUE34_RS02590 (529111)	529111..530475	-	1365	WP_032302573.1	MFS transporter	-
QUE34_RS02595 (530892)	530892..531827	+	936	Protein_508	tetratricopeptide repeat protein	-
QUE34_RS02600 (531883)	531883..532425	-	543	WP_105268434.1	GNAT family N-acetyltransferase	Toxin
QUE34_RS02605 (532409)	532409..532720	-	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
QUE34_RS02610 (532905)	532905..533795	-	891	WP_000971336.1	heme o synthase	-
QUE34_RS02615 (533807)	533807..534136	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QUE34_RS02620 (534136)	534136..534750	-	615	WP_000179807.1	cytochrome o ubiquinol oxidase subunit III	-
QUE34_RS02625 (534740)	534740..536731	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QUE34_RS02630 (536753)	536753..537700	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19960.31 Da
• Isoelectric Point: 9.1699

>T40826 WP_105268434.1 NZ_AP025754:c532425-531883 [Escherichia sp. KS167_9B]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGKPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T40826 NZ_AP025754:c532425-531883 [Escherichia sp. KS167_9B]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAAACCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGTCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT40826 WP_001353405.1 NZ_AP025754:c532720-532409 [Escherichia sp. KS167_9B]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT40826 NZ_AP025754:c532720-532409 [Escherichia sp. KS167_9B]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A829G7G3