Detailed information of TA system TA040825

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 352879..354568
• Replicon: chromosome
• Accession: NZ_AP025754
• Organism: Escherichia sp. KS167_9B strain KS-P079

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A2K3TY15
• Locus tag: QUE34_RS01795
• Protein ID: WP_032302488.1
• Coordinates: 353648..354568 (+)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: QUE34_RS01790
• Protein ID: WP_198600491.1
• Coordinates: 352879..353661 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE34_RS01770 (348188)	348188..348390	-	203	Protein_343	integrase	-
QUE34_RS01775 (348437)	348437..348539	-	103	Protein_344	helix-turn-helix domain-containing protein	-
QUE34_RS01780 (348536)	348536..349006	-	471	WP_000201186.1	hypothetical protein	-
QUE34_RS01785 (349353)	349353..352562	-	3210	WP_103253298.1	hypothetical protein	-
QUE34_RS01790 (352879)	352879..353661	+	783	WP_198600491.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
QUE34_RS01795 (353648)	353648..354568	+	921	WP_032302488.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
QUE34_RS01800 (354731)	354731..354823	-	93	Protein_349	small membrane protein YkgR	-
QUE34_RS01805 (355029)	355029..355913	-	885	WP_001119037.1	LysR family transcriptional regulator	-
QUE34_RS01810 (356178)	356178..357236	+	1059	WP_123058987.1	alpha/beta hydrolase	-
QUE34_RS01815 (357378)	357378..358505	+	1128	WP_123059891.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	303897..355913	52016

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34721.06 Da
• Isoelectric Point: 6.9552

>T40825 WP_032302488.1 NZ_AP025754:353648-354568 [Escherichia sp. KS167_9B]
MDLRDSYTRQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKTFADRDTDLTAINDSLKRITASLNSK
PGITAIRQENKTDEKRIIVNTADAQIKIEVSPVWRGLLLPPAEMSVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHFPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T40825 NZ_AP025754:353648-354568 [Escherichia sp. KS167_9B]
ATGGATCTGCGTGATAGCTATACCCGGCAGGTCAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAGTCCTGTTT
CGCATTAAAAGGCGGCACCGCAATCAATTTGTTTGTGCAGGATTTTCCCCGTCTGTCGGTGGATATCGATCTGGCGTATA
AGACTTTTGCGGATCGCGATACCGATTTGACGGCGATCAACGACTCTTTGAAGAGAATTACTGCATCGCTGAACAGTAAA
CCGGGGATTACCGCGATTCGGCAGGAAAATAAAACGGATGAAAAGCGCATCATTGTGAATACGGCTGATGCCCAGATTAA
GATCGAGGTTAGTCCGGTCTGGCGTGGGCTGTTGTTGCCTCCGGCAGAAATGTCGGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACTATGAGTGTCGTTTCGCTGGCGGATCTTTATGGCGGTAAAATCTGCGCAGCGCTTGATCGTCAGCAC
CCGCGCGATCTGTTTGATGTGCTGAATATGCTGGAGAAACCGGGACTGATGCGGGAGATCTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGCCCCATTGCCGAGTTGCTGGCCCCAAACTGGGATACAGAGCGGATCACGACGCTGTATGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTGCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATCTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATTTTCCGGCTATTCGCTGGAAGCAACGTAATCTGGCGGTGCTGAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGCGTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29205.45 Da
• Isoelectric Point: 7.8414

>AT40825 WP_198600491.1 NZ_AP025754:352879-353661 [Escherichia sp. KS167_9B]
MPNYLNWLMQHTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYFRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVTSLTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPCEFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT40825 NZ_AP025754:352879-353661 [Escherichia sp. KS167_9B]
TTGCCAAACTATCTAAACTGGCTGATGCAGCATACACTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAACGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTTCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTTAA
GCTGCCGGGATGGTTTGCTGCCTTCGAACAATCTGCAACATTTATCACGCTGTATACATCGGGGCTTACCGTAGATGTAA
CGTCATTGACTACGGAACTGAAAATTGGCGACCGCCAGTTAGCAGGCAGTGTTCCGGAACTTGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGTTGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTGGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCATGCGAATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A2K3TY15