Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 58784..59334 Replicon chromosome
Accession NZ_AP025754
Organism Escherichia sp. KS167_9B strain KS-P079

Toxin (Protein)


Gene name ccdB Uniprot ID A0A2K3TU88
Locus tag QUE34_RS00270 Protein ID WP_032302363.1
Coordinates 59020..59334 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A2K3TUD9
Locus tag QUE34_RS00265 Protein ID WP_032302360.1
Coordinates 58784..59017 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE34_RS00240 (53866) 53866..54153 + 288 WP_000203741.1 ferredoxin-like protein FixX -
QUE34_RS00245 (54213) 54213..55544 + 1332 WP_032302356.1 MFS transporter -
QUE34_RS00250 (55652) 55652..56182 + 531 WP_032183187.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
QUE34_RS00255 (56175) 56175..58027 + 1853 Protein_50 glutathione-regulated potassium-efflux system protein KefC -
QUE34_RS00260 (58219) 58219..58698 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
QUE34_RS00265 (58784) 58784..59017 + 234 WP_032302360.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
QUE34_RS00270 (59020) 59020..59334 + 315 WP_032302363.1 CcdB family protein Toxin
QUE34_RS00275 (59331) 59331..60179 - 849 WP_000257193.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
QUE34_RS00280 (60186) 60186..60563 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
QUE34_RS00285 (60566) 60566..61387 - 822 WP_001065391.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
QUE34_RS00290 (61384) 61384..62373 - 990 WP_032302365.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
QUE34_RS00295 (62373) 62373..63659 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11689.63 Da        Isoelectric Point: 8.0666

>T40824 WP_032302363.1 NZ_AP025754:59020-59334 [Escherichia sp. KS167_9B]
MQFTVYRSRGRNAALPFVIDVTSDIIGEINRRIVNPLTPIERFSHIRPPERLNPILLLVDGKEYVLMTHETATVPVNTLG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T40824 NZ_AP025754:59020-59334 [Escherichia sp. KS167_9B]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTGCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTAATCCATTAACACCTATTGAACGATTTAGCCATATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACACGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACTTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8557.55 Da        Isoelectric Point: 5.8062

>AT40824 WP_032302360.1 NZ_AP025754:58784-59017 [Escherichia sp. KS167_9B]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRNF

Download         Length: 234 bp

>AT40824 NZ_AP025754:58784-59017 [Escherichia sp. KS167_9B]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAATG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAACTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K3TU88

Antitoxin

Source ID Structure
AlphaFold DB A0A2K3TUD9

References