Detailed information of TA system TA040819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RelE-RHH
• Location: 1988..2535
• Replicon: plasmid pla2
• Accession: NZ_AP025753
• Organism: Escherichia sp. HH154_1D strain HH-P049

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: QUE53_RS27560
• Protein ID: WP_013009346.1
• Coordinates: 2257..2535 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: E0WH52
• Locus tag: QUE53_RS27555
• Protein ID: WP_000079932.1
• Coordinates: 1988..2257 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE53_RS27545 (46)	46..1077	+	1032	WP_000907868.1	plasmid replication initiator RepA	-
QUE53_RS27550 (1775)	1775..1933	+	159	WP_000829588.1	hypothetical protein	-
QUE53_RS27555 (1988)	1988..2257	+	270	WP_000079932.1	type II toxin-antitoxin system antitoxin YacA	Antitoxin
QUE53_RS27560 (2257)	2257..2535	+	279	WP_013009346.1	type II toxin-antitoxin system toxin YacB	Toxin
QUE53_RS27565 (2731)	2731..3027	-	297	WP_001419651.1	hypothetical protein	-
QUE53_RS27570 (3372)	3372..3959	+	588	WP_286347576.1	serine acetyltransferase	-
QUE53_RS27575 (4241)	4241..4834	-	594	WP_001401779.1	ProQ/FINO family protein	-
QUE53_RS27580 (5188)	5188..6534	+	1347	WP_001132032.1	protein YagA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..91132	91132

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10748.35 Da
• Isoelectric Point: 8.4615

>T40819 WP_013009346.1 NZ_AP025753:2257-2535 [Escherichia sp. HH154_1D]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
VLRVLHTAQKWP

Nucleotide

• Length: 279 bp

>T40819 NZ_AP025753:2257-2535 [Escherichia sp. HH154_1D]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAACGGTCGTG
TGGAAGGAACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
GTCCTGCGCGTGTTGCATACCGCACAGAAGTGGCCATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10268.58 Da
• Isoelectric Point: 7.2055

>AT40819 WP_000079932.1 NZ_AP025753:1988-2257 [Escherichia sp. HH154_1D]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVSDK

Nucleotide

• Length: 270 bp

>AT40819 NZ_AP025753:1988-2257 [Escherichia sp. HH154_1D]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCTGCCGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTTCCGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A5U8RN22