Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 140940..141499 Replicon plasmid pla1
Accession NZ_AP025752
Organism Escherichia sp. HH154_1D strain HH-P049

Toxin (Protein)


Gene name relE Uniprot ID A0A0B1JMW5
Locus tag QUE53_RS27150 Protein ID WP_000421249.1
Coordinates 140940..141215 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0B1JM87
Locus tag QUE53_RS27155 Protein ID WP_001355337.1
Coordinates 141215..141499 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE53_RS27110 (136586) 136586..137049 - 464 Protein_171 DUF1380 family protein -
QUE53_RS27115 (137009) 137009..137230 - 222 WP_001104895.1 hypothetical protein -
QUE53_RS27120 (137231) 137231..137914 - 684 WP_000085877.1 DNA methylase -
QUE53_RS27125 (137993) 137993..138289 - 297 WP_001364075.1 hypothetical protein -
QUE53_RS27130 (138432) 138432..139394 + 963 WP_105279257.1 plasmid segregation protein ParM -
QUE53_RS27135 (139397) 139397..139747 + 351 WP_024166183.1 plasmid partitioning/stability family protein -
QUE53_RS27140 (139854) 139854..140213 + 360 WP_000765123.1 hypothetical protein -
QUE53_RS27145 (140631) 140631..140834 - 204 Protein_178 hypothetical protein -
QUE53_RS27150 (140940) 140940..141215 - 276 WP_000421249.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QUE53_RS27155 (141215) 141215..141499 - 285 WP_001355337.1 ribbon-helix-helix protein, CopG family Antitoxin
QUE53_RS27160 (141811) 141811..142047 + 237 WP_024221845.1 hypothetical protein -
QUE53_RS27165 (142220) 142220..142625 + 406 Protein_182 hypothetical protein -
QUE53_RS27170 (142632) 142632..142853 + 222 Protein_183 integrase core domain-containing protein -
QUE53_RS27175 (142999) 142999..143589 + 591 WP_001355339.1 hypothetical protein -
QUE53_RS27180 (145274) 145274..146146 + 873 Protein_185 IS3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyD / hlyD / hlyB / hlyB / hlyA / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / espP 1..220055 220055


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10722.42 Da        Isoelectric Point: 9.9324

>T40818 WP_000421249.1 NZ_AP025752:c141215-140940 [Escherichia sp. HH154_1D]
MELKWTSKALSDLARLYDFLALASKPAAARTVQSLTQAPIILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T40818 NZ_AP025752:c141215-140940 [Escherichia sp. HH154_1D]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGCGCTGGCCAGTAAACCAGC
TGCAGCCAGAACGGTGCAGTCCCTGACACAGGCACCGATCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGTGAGTACGAAATCCGTTACGAACTTACTGGTCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10550.99 Da        Isoelectric Point: 5.6706

>AT40818 WP_001355337.1 NZ_AP025752:c141499-141215 [Escherichia sp. HH154_1D]
MQMKNNTAQATKVITAHVPLLMADKVDQMAARLERSRGWIIKQALSAWIAQEEERNRLTLEALDDVTSGRVIDHQAVQAW
ADSLDTDNSLPVPR

Download         Length: 285 bp

>AT40818 NZ_AP025752:c141499-141215 [Escherichia sp. HH154_1D]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACTTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGGTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGATTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCACTGGACGATGTGACATCCGGACGGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCGATACTGACAATTCGTTACCGGTGCCTCGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0B1JMW5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B1JM87

References