Detailed information of TA system TA040814

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 92269..92894
• Replicon: plasmid pla1
• Accession: NZ_AP025752
• Organism: Escherichia sp. HH154_1D strain HH-P049

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QUE53_RS26805
• Protein ID: WP_000911316.1
• Coordinates: 92496..92894 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8H9EG71
• Locus tag: QUE53_RS26800
• Protein ID: WP_000450526.1
• Coordinates: 92269..92496 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE53_RS26800 (92269)	92269..92496	+	228	WP_000450526.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QUE53_RS26805 (92496)	92496..92894	+	399	WP_000911316.1	type II toxin-antitoxin system VapC family toxin	Toxin
QUE53_RS26810 (92903)	92903..95047	-	2145	WP_000009375.1	type IV conjugative transfer system coupling protein TraD	-
QUE53_RS26815 (95103)	95103..95833	-	731	Protein_112	hypothetical protein	-
QUE53_RS26820 (96068)	96068..96679	-	612	WP_000908443.1	hypothetical protein	-
QUE53_RS26825 (96751)	96751..97482	-	732	WP_001364068.1	conjugal transfer complement resistance protein TraT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyD / hlyD / hlyB / hlyB / hlyA / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / espP	1..220055	220055

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14915.18 Da
• Isoelectric Point: 7.8605

>T40814 WP_000911316.1 NZ_AP025752:92496-92894 [Escherichia sp. HH154_1D]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T40814 NZ_AP025752:92496-92894 [Escherichia sp. HH154_1D]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACAATAAAGAACAAGCCCGCCAGTGTCAGAGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGCTCTGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGCAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCAGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGACCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGCGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.53 Da
• Isoelectric Point: 4.5358

>AT40814 WP_000450526.1 NZ_AP025752:92269-92496 [Escherichia sp. HH154_1D]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT40814 NZ_AP025752:92269-92496 [Escherichia sp. HH154_1D]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGGCTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGTATCATTACGCCAGCCGGAGAGTCATGGGATGAATGGTTCGACGGAA
ACAGCGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA