Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 40444..40966 Replicon plasmid pla1
Accession NZ_AP025752
Organism Escherichia sp. HH154_1D strain HH-P049

Toxin (Protein)


Gene name relE Uniprot ID A0A2A6Q014
Locus tag QUE53_RS26460 Protein ID WP_000220564.1
Coordinates 40444..40725 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0B0W3C1
Locus tag QUE53_RS26465 Protein ID WP_000121742.1
Coordinates 40715..40966 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE53_RS26435 (35485) 35485..36667 + 1183 Protein_36 IS21 family transposase -
QUE53_RS26440 (36664) 36664..37418 + 755 Protein_37 IS21-like element ISEc10 family helper ATPase IstB -
QUE53_RS26445 (37483) 37483..37965 + 483 Protein_38 IS21-like element helper ATPase IstB -
QUE53_RS26450 (38033) 38033..38695 + 663 Protein_39 IS3 family transposase -
QUE53_RS26455 (38753) 38753..39960 - 1208 Protein_40 IS256 family transposase -
QUE53_RS26460 (40444) 40444..40725 - 282 WP_000220564.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QUE53_RS26465 (40715) 40715..40966 - 252 WP_000121742.1 hypothetical protein Antitoxin
QUE53_RS26470 (41242) 41242..42261 - 1020 WP_024262278.1 IS110 family transposase -
QUE53_RS26475 (43638) 43638..43639 - 2 WP_097638450.1 IS3 family transposase -
QUE53_RS26480 (43971) 43971..44774 - 804 Protein_45 IS256-like element IS1414 family transposase -
QUE53_RS26485 (44946) 44946..45566 - 621 WP_000269722.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyD / hlyD / hlyB / hlyB / hlyA / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / espP 1..220055 220055
- inside IScluster/Tn - - 33160..69697 36537


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11009.78 Da        Isoelectric Point: 10.5938

>T40808 WP_000220564.1 NZ_AP025752:c40725-40444 [Escherichia sp. HH154_1D]
MTYELAFDRRALKEWQKLGHTIREQFKKKLSERLENPRAPAARLHGHADRYKIKLRSSGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T40808 NZ_AP025752:c40725-40444 [Escherichia sp. HH154_1D]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGTCAGAACGGCTGGAAAATCCACGCGCACCTGCAGCCCGATTACATGGTCATGCTGATCGTTATAAAATCA
AACTTCGTTCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGAGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9181.47 Da        Isoelectric Point: 4.2958

>AT40808 WP_000121742.1 NZ_AP025752:c40966-40715 [Escherichia sp. HH154_1D]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKKPEIVKVSL
DDL

Download         Length: 252 bp

>AT40808 NZ_AP025752:c40966-40715 [Escherichia sp. HH154_1D]
ATGAGTTATCAAATCCTGACAACCACAGCGGCCAGTATTACTGACCTAAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCAATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAAACCGGAAATTGTGAAGGTTAGCCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A6Q014

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0W3C1

References