Detailed information of TA system TA040807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 4337871..4338483
• Replicon: chromosome
• Accession: NZ_AP025751
• Organism: Escherichia sp. HH154_1D strain HH-P049

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: QUE53_RS21570
• Protein ID: WP_000833473.1
• Coordinates: 4338298..4338483 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: QUE53_RS21565
• Protein ID: WP_000499744.1
• Coordinates: 4337871..4338281 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE53_RS21540 (4334077)	4334077..4334817	-	741	WP_000906829.1	MipA/OmpV family protein	-
QUE53_RS21545 (4334941)	4334941..4335915	+	975	WP_000164020.1	LysR family transcriptional regulator	-
QUE53_RS21550 (4335912)	4335912..4337048	-	1137	WP_000364886.1	HlyD family secretion protein	-
QUE53_RS21555 (4337054)	4337054..4337377	-	324	WP_000478187.1	DUF3302 domain-containing protein	-
QUE53_RS21560 (4337560)	4337560..4337874	+	315	WP_001064341.1	hypothetical protein	-
QUE53_RS21565 (4337871)	4337871..4338281	-	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
QUE53_RS21570 (4338298)	4338298..4338483	-	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
QUE53_RS21575 (4338761)	4338761..4340299	-	1539	WP_000183976.1	aldehyde dehydrogenase AldB	-
QUE53_RS21580 (4340500)	4340500..4341651	-	1152	WP_000741501.1	L-threonine dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T40807 WP_000833473.1 NZ_AP025751:c4338483-4338298 [Escherichia sp. HH154_1D]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T40807 NZ_AP025751:c4338483-4338298 [Escherichia sp. HH154_1D]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGTAGTCATCATCAGTT
CCGCCACCCTGTCCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT40807 WP_000499744.1 NZ_AP025751:c4338281-4337871 [Escherichia sp. HH154_1D]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT40807 NZ_AP025751:c4338281-4337871 [Escherichia sp. HH154_1D]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGATGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9