Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3573473..3574127 Replicon chromosome
Accession NZ_AP025751
Organism Escherichia sp. HH154_1D strain HH-P049

Toxin (Protein)


Gene name cptA Uniprot ID F0JPE9
Locus tag QUE53_RS17660 Protein ID WP_000244774.1
Coordinates 3573473..3573880 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUE53_RS17665 Protein ID WP_000354046.1
Coordinates 3573861..3574127 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE53_RS17640 (3569430) 3569430..3571163 - 1734 WP_286347496.1 single-stranded-DNA-specific exonuclease RecJ -
QUE53_RS17645 (3571169) 3571169..3571879 - 711 WP_000715223.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUE53_RS17650 (3571904) 3571904..3572800 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QUE53_RS17655 (3572912) 3572912..3573433 + 522 WP_001055872.1 flavodoxin FldB -
QUE53_RS17660 (3573473) 3573473..3573880 - 408 WP_000244774.1 protein YgfX Toxin
QUE53_RS17665 (3573861) 3573861..3574127 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUE53_RS17670 (3574379) 3574379..3575359 + 981 WP_000886106.1 tRNA-modifying protein YgfZ -
QUE53_RS17675 (3575555) 3575555..3576214 - 660 WP_000250283.1 hemolysin III family protein -
QUE53_RS17680 (3576378) 3576378..3576689 - 312 WP_001182962.1 N(4)-acetylcytidine aminohydrolase -
QUE53_RS17685 (3576734) 3576734..3578167 + 1434 WP_001354958.1 6-phospho-beta-glucosidase BglA -
QUE53_RS17690 (3578224) 3578224..3578967 - 744 WP_000150658.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16078.05 Da        Isoelectric Point: 11.2511

>T40804 WP_000244774.1 NZ_AP025751:c3573880-3573473 [Escherichia sp. HH154_1D]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T40804 NZ_AP025751:c3573880-3573473 [Escherichia sp. HH154_1D]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGTTAAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGTTGCGTTTACGTTCTGATAGCGGTAAACGTCA
GCACTTGTGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT40804 WP_000354046.1 NZ_AP025751:c3574127-3573861 [Escherichia sp. HH154_1D]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT40804 NZ_AP025751:c3574127-3573861 [Escherichia sp. HH154_1D]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGTGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L6ZX35

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References