Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 532062..532680 Replicon chromosome
Accession NZ_AP025751
Organism Escherichia sp. HH154_1D strain HH-P049

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QUE53_RS02455 Protein ID WP_001291435.1
Coordinates 532062..532280 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QUE53_RS02460 Protein ID WP_000344800.1
Coordinates 532306..532680 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE53_RS02420 (527351) 527351..527923 + 573 WP_000779831.1 YbaY family lipoprotein -
QUE53_RS02425 (527954) 527954..528265 - 312 WP_000409907.1 MGMT family protein -
QUE53_RS02435 (528644) 528644..528997 + 354 WP_000878140.1 DUF1428 domain-containing protein -
QUE53_RS02440 (529039) 529039..530589 - 1551 WP_001355176.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QUE53_RS02445 (530753) 530753..531223 - 471 WP_000136192.1 YlaC family protein -
QUE53_RS02450 (531339) 531339..531890 - 552 WP_000102575.1 maltose O-acetyltransferase -
QUE53_RS02455 (532062) 532062..532280 - 219 WP_001291435.1 HHA domain-containing protein Toxin
QUE53_RS02460 (532306) 532306..532680 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QUE53_RS02465 (533227) 533227..536376 - 3150 WP_001132492.1 efflux RND transporter permease AcrB -
QUE53_RS02470 (536399) 536399..537592 - 1194 WP_001353399.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T40782 WP_001291435.1 NZ_AP025751:c532280-532062 [Escherichia sp. HH154_1D]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T40782 NZ_AP025751:c532280-532062 [Escherichia sp. HH154_1D]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTGCGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT40782 WP_000344800.1 NZ_AP025751:c532680-532306 [Escherichia sp. HH154_1D]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT40782 NZ_AP025751:c532680-532306 [Escherichia sp. HH154_1D]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGATTGCCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAAAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References