Detailed information of TA system TA040781

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 497751..498588
• Replicon: chromosome
• Accession: NZ_AP025751
• Organism: Escherichia sp. HH154_1D strain HH-P049

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: V0SED1
• Locus tag: QUE53_RS02285
• Protein ID: WP_000227787.1
• Coordinates: 497751..498293 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: QUE53_RS02290
• Protein ID: WP_001353405.1
• Coordinates: 498277..498588 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE53_RS02265 (493281)	493281..494192	-	912	WP_000705835.1	2-dehydropantoate 2-reductase	-
QUE53_RS02270 (494360)	494360..494851	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
QUE53_RS02275 (494979)	494979..496343	-	1365	WP_001000949.1	MFS transporter	-
QUE53_RS02280 (496760)	496760..497695	+	936	Protein_447	tetratricopeptide repeat protein	-
QUE53_RS02285 (497751)	497751..498293	-	543	WP_000227787.1	GNAT family N-acetyltransferase	Toxin
QUE53_RS02290 (498277)	498277..498588	-	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
QUE53_RS02295 (498773)	498773..499663	-	891	WP_000971336.1	heme o synthase	-
QUE53_RS02300 (499675)	499675..500004	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QUE53_RS02305 (500004)	500004..500618	-	615	WP_000179807.1	cytochrome o ubiquinol oxidase subunit III	-
QUE53_RS02310 (500608)	500608..502599	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QUE53_RS02315 (502621)	502621..503568	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19946.24 Da
• Isoelectric Point: 8.8951

>T40781 WP_000227787.1 NZ_AP025751:c498293-497751 [Escherichia sp. HH154_1D]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T40781 NZ_AP025751:c498293-497751 [Escherichia sp. HH154_1D]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGTCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT40781 WP_001353405.1 NZ_AP025751:c498588-498277 [Escherichia sp. HH154_1D]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT40781 NZ_AP025751:c498588-498277 [Escherichia sp. HH154_1D]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A829GE43

Antitoxin

• AlphaFold DB: A0A829G7G3