Detailed information of TA system TA040778

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 120987..121546
• Replicon: plasmid pla1
• Accession: NZ_AP025748
• Organism: Escherichia sp. HH091_1A strain HH-P024

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0B1JMW5
• Locus tag: QUF14_RS27895
• Protein ID: WP_000421249.1
• Coordinates: 120987..121262 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0B1JM87
• Locus tag: QUF14_RS27900
• Protein ID: WP_001355337.1
• Coordinates: 121262..121546 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF14_RS27855 (116728)	116728..117191	-	464	Protein_143	DUF1380 family protein	-
QUF14_RS27860 (117151)	117151..117372	-	222	WP_001104895.1	hypothetical protein	-
QUF14_RS27865 (117373)	117373..118056	-	684	WP_000085877.1	DNA methylase	-
QUF14_RS27870 (118135)	118135..118464	-	330	WP_047088698.1	hypothetical protein	-
QUF14_RS27875 (118479)	118479..119441	+	963	WP_000959884.1	plasmid segregation protein ParM	-
QUF14_RS27880 (119444)	119444..119794	+	351	WP_024166183.1	plasmid partitioning/stability family protein	-
QUF14_RS27885 (119901)	119901..120260	+	360	WP_000765123.1	hypothetical protein	-
QUF14_RS27890 (120678)	120678..120881	-	204	Protein_150	hypothetical protein	-
QUF14_RS27895 (120987)	120987..121262	-	276	WP_000421249.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUF14_RS27900 (121262)	121262..121546	-	285	WP_001355337.1	ribbon-helix-helix protein, CopG family	Antitoxin
QUF14_RS27905 (121927)	121927..122411	-	485	Protein_153	IS3 family transposase	-
QUF14_RS27910 (122425)	122425..123425	+	1001	Protein_154	IS21 family transposase	-
QUF14_RS27915 (123422)	123422..124194	+	773	Protein_155	IS21-like element helper ATPase IstB	-
QUF14_RS27920 (124500)	124500..124676	+	177	WP_157839835.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	estIa / estIa / hlyC / hlyA / hlyB / hlyD / exeG / exeE / stcE / faeJ / faeI / faeH / faeF / faeE / faeD / faeC	1..225721	225721

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10722.42 Da
• Isoelectric Point: 9.9324

>T40778 WP_000421249.1 NZ_AP025748:c121262-120987 [Escherichia sp. HH091_1A]
MELKWTSKALSDLARLYDFLALASKPAAARTVQSLTQAPIILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T40778 NZ_AP025748:c121262-120987 [Escherichia sp. HH091_1A]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGCGCTGGCCAGTAAACCAGC
TGCAGCCAGAACGGTGCAGTCCCTGACACAGGCACCGATCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGTGAGTACGAAATCCGTTACGAACTTACTGGTCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10550.99 Da
• Isoelectric Point: 5.6706

>AT40778 WP_001355337.1 NZ_AP025748:c121546-121262 [Escherichia sp. HH091_1A]
MQMKNNTAQATKVITAHVPLLMADKVDQMAARLERSRGWIIKQALSAWIAQEEERNRLTLEALDDVTSGRVIDHQAVQAW
ADSLDTDNSLPVPR

Nucleotide

• Length: 285 bp

>AT40778 NZ_AP025748:c121546-121262 [Escherichia sp. HH091_1A]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACTTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGGTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGATTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCACTGGACGATGTGACATCCGGACGGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCGATACTGACAATTCGTTACCGGTGCCTCGCTGA

Structures

Toxin

• AlphaFold DB: A0A0B1JMW5

Antitoxin

• AlphaFold DB: A0A0B1JM87