Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5061976..5062473 Replicon chromosome
Accession NZ_AP025747
Organism Escherichia sp. HH091_1A strain HH-P024

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QUF14_RS24985 Protein ID WP_001173343.1
Coordinates 5062300..5062473 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QUF14_RS24980 Protein ID WP_000398619.1
Coordinates 5061976..5062272 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF14_RS24965 (5059981) 5059981..5060127 + 147 Protein_4867 4Fe-4S mono-cluster protein YjdI -
QUF14_RS24970 (5060139) 5060139..5060411 + 273 WP_000405654.1 GNAT family N-acetyltransferase -
QUF14_RS24975 (5060627) 5060627..5061855 + 1229 WP_097494069.1 IS3-like element IS2 family transposase -
QUF14_RS24980 (5061976) 5061976..5062272 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUF14_RS24985 (5062300) 5062300..5062473 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QUF14_RS24990 (5062592) 5062592..5064109 - 1518 WP_000003806.1 lysine--tRNA ligase -
QUF14_RS24995 (5064346) 5064346..5065803 - 1458 WP_000856811.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 5037476..5071326 33850


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T40766 WP_001173343.1 NZ_AP025747:5062300-5062473 [Escherichia sp. HH091_1A]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T40766 NZ_AP025747:5062300-5062473 [Escherichia sp. HH091_1A]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGCCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT40766 WP_000398619.1 NZ_AP025747:5061976-5062272 [Escherichia sp. HH091_1A]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT40766 NZ_AP025747:5061976-5062272 [Escherichia sp. HH091_1A]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References