Detailed information of TA system TA040740

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 528271..529108
• Replicon: chromosome
• Accession: NZ_AP025747
• Organism: Escherichia sp. HH091_1A strain HH-P024

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: QUF14_RS02465
• Protein ID: WP_000227789.1
• Coordinates: 528271..528813 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: QUF14_RS02470
• Protein ID: WP_001353405.1
• Coordinates: 528797..529108 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF14_RS02445 (523801)	523801..524712	-	912	WP_000705835.1	2-dehydropantoate 2-reductase	-
QUF14_RS02450 (524880)	524880..525371	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
QUF14_RS02455 (525499)	525499..526863	-	1365	WP_001000980.1	MFS transporter	-
QUF14_RS02460 (527280)	527280..528215	+	936	WP_100733868.1	tetratricopeptide repeat protein	-
QUF14_RS02465 (528271)	528271..528813	-	543	WP_000227789.1	GNAT family N-acetyltransferase	Toxin
QUF14_RS02470 (528797)	528797..529108	-	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
QUF14_RS02475 (529293)	529293..530183	-	891	WP_000971336.1	heme o synthase	-
QUF14_RS02480 (530195)	530195..530524	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QUF14_RS02485 (530524)	530524..531138	-	615	WP_000179807.1	cytochrome o ubiquinol oxidase subunit III	-
QUF14_RS02490 (531128)	531128..533119	-	1992	WP_000467181.1	cytochrome o ubiquinol oxidase subunit I	-
QUF14_RS02495 (533141)	533141..534088	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19974.25 Da
• Isoelectric Point: 8.8985

>T40740 WP_000227789.1 NZ_AP025747:c528813-528271 [Escherichia sp. HH091_1A]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLER
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T40740 NZ_AP025747:c528813-528271 [Escherichia sp. HH091_1A]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAGA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGTCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT40740 WP_001353405.1 NZ_AP025747:c529108-528797 [Escherichia sp. HH091_1A]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT40740 NZ_AP025747:c529108-528797 [Escherichia sp. HH091_1A]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Antitoxin

• AlphaFold DB: A0A829G7G3