Detailed information of TA system TA040737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 140949..141508
• Replicon: plasmid pla1
• Accession: NZ_AP025742
• Organism: Escherichia sp. 10290

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0B1JMW5
• Locus tag: QUE74_RS28200
• Protein ID: WP_000421249.1
• Coordinates: 140949..141224 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0B1JM87
• Locus tag: QUE74_RS28205
• Protein ID: WP_001355337.1
• Coordinates: 141224..141508 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE74_RS28160 (136595)	136595..137058	-	464	Protein_171	DUF1380 family protein	-
QUE74_RS28165 (137018)	137018..137239	-	222	WP_001104895.1	hypothetical protein	-
QUE74_RS28170 (137240)	137240..137923	-	684	WP_000085877.1	DNA methylase	-
QUE74_RS28175 (138002)	138002..138298	-	297	WP_001364075.1	hypothetical protein	-
QUE74_RS28180 (138441)	138441..139403	+	963	WP_105279257.1	plasmid segregation protein ParM	-
QUE74_RS28185 (139406)	139406..139756	+	351	WP_024166183.1	plasmid partitioning/stability family protein	-
QUE74_RS28190 (139863)	139863..140222	+	360	WP_000765123.1	hypothetical protein	-
QUE74_RS28195 (140640)	140640..140843	-	204	Protein_178	hypothetical protein	-
QUE74_RS28200 (140949)	140949..141224	-	276	WP_000421249.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE74_RS28205 (141224)	141224..141508	-	285	WP_001355337.1	ribbon-helix-helix protein, CopG family	Antitoxin
QUE74_RS28210 (141901)	141901..142122	-	222	WP_001261037.1	MarR family transcriptional regulator	-
QUE74_RS28215 (142229)	142229..142634	+	406	Protein_182	hypothetical protein	-
QUE74_RS28220 (142641)	142641..142862	+	222	Protein_183	integrase core domain-containing protein	-
QUE74_RS28225 (143008)	143008..143598	+	591	WP_001355339.1	hypothetical protein	-
QUE74_RS28230 (145283)	145283..146155	+	873	Protein_185	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyD / hlyD / hlyB / hlyB / hlyA / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / espP	1..224339	224339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10722.42 Da
• Isoelectric Point: 9.9324

>T40737 WP_000421249.1 NZ_AP025742:c141224-140949 [Escherichia sp. 10290]
MELKWTSKALSDLARLYDFLALASKPAAARTVQSLTQAPIILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T40737 NZ_AP025742:c141224-140949 [Escherichia sp. 10290]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGCGCTGGCCAGTAAACCAGC
TGCAGCCAGAACGGTGCAGTCCCTGACACAGGCACCGATCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGTGAGTACGAAATCCGTTACGAACTTACTGGTCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10550.99 Da
• Isoelectric Point: 5.6706

>AT40737 WP_001355337.1 NZ_AP025742:c141508-141224 [Escherichia sp. 10290]
MQMKNNTAQATKVITAHVPLLMADKVDQMAARLERSRGWIIKQALSAWIAQEEERNRLTLEALDDVTSGRVIDHQAVQAW
ADSLDTDNSLPVPR

Nucleotide

• Length: 285 bp

>AT40737 NZ_AP025742:c141508-141224 [Escherichia sp. 10290]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACTTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGGTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGATTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCACTGGACGATGTGACATCCGGACGGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCGATACTGACAATTCGTTACCGGTGCCTCGCTGA

Structures

Toxin

• AlphaFold DB: A0A0B1JMW5

Antitoxin

• AlphaFold DB: A0A0B1JM87