Detailed information of TA system TA040733

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 92278..92903
• Replicon: plasmid pla1
• Accession: NZ_AP025742
• Organism: Escherichia sp. 10290

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QUE74_RS27855
• Protein ID: WP_000911316.1
• Coordinates: 92505..92903 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8H9EG71
• Locus tag: QUE74_RS27850
• Protein ID: WP_000450526.1
• Coordinates: 92278..92505 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE74_RS27850 (92278)	92278..92505	+	228	WP_000450526.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QUE74_RS27855 (92505)	92505..92903	+	399	WP_000911316.1	type II toxin-antitoxin system VapC family toxin	Toxin
QUE74_RS27860 (92912)	92912..95056	-	2145	WP_000009375.1	type IV conjugative transfer system coupling protein TraD	-
QUE74_RS27865 (95112)	95112..95842	-	731	Protein_112	hypothetical protein	-
QUE74_RS27870 (96077)	96077..96688	-	612	WP_000908443.1	hypothetical protein	-
QUE74_RS27875 (96760)	96760..97491	-	732	WP_001364068.1	conjugal transfer complement resistance protein TraT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyD / hlyD / hlyB / hlyB / hlyA / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / espP	1..224339	224339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14915.18 Da
• Isoelectric Point: 7.8605

>T40733 WP_000911316.1 NZ_AP025742:92505-92903 [Escherichia sp. 10290]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVDGLRIEDWS

Nucleotide

• Length: 399 bp

>T40733 NZ_AP025742:92505-92903 [Escherichia sp. 10290]
ATGCTGAAGTTTATGCTCGATACCAACATCTGCATTTTCACAATAAAGAACAAGCCCGCCAGTGTCAGAGAACGTTTTAA
CCTGAACCAGGGGAGAATGTGTATCAGCTCTGTCACCCTGATGGAGCTGATATATGGTGCAGAAAAAAGCCAGATGCCTG
AACGCAATCTCGCTGTGATCGAGGGATTTGTTTCCCGCATTGATGTTCTGGATTACGACGCTGCAGCAGCCACACACACC
GGCCAGATAAGAGCAGAACTTGCCCGTCAGGGACGCCCTGTCGGACCATTTGATCAAATGATCGCAGGTCATGCCCGCAG
TCGGGGACTGATTATTGTGACTAATAACACCCGGGAATTTGAACGTGTGGACGGTCTGCGAATTGAAGACTGGAGCTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.53 Da
• Isoelectric Point: 4.5358

>AT40733 WP_000450526.1 NZ_AP025742:92278-92505 [Escherichia sp. 10290]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGESWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT40733 NZ_AP025742:92278-92505 [Escherichia sp. 10290]
ATGGAAACCACCGTATTTCTCAGCAACCGCAGCCAGGCGGTCAGGCTGCCAAAAGCGGTTGCATTGCCGGAAAACGTAAA
ACGCGTTGAAGTGATTGCTGTCGGACGAACCCGTATCATTACGCCAGCCGGAGAGTCATGGGATGAATGGTTCGACGGAA
ACAGCGTCAGCGCTGATTTTATGGATAACAGGGAACAGCCCGGCATGCAGGAAAGGGAGTCATTCTGA