Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 40448..40970 Replicon plasmid pla1
Accession NZ_AP025742
Organism Escherichia sp. 10290

Toxin (Protein)


Gene name relE Uniprot ID A0A2A6Q014
Locus tag QUE74_RS27510 Protein ID WP_000220564.1
Coordinates 40448..40729 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0B0W3C1
Locus tag QUE74_RS27515 Protein ID WP_000121742.1
Coordinates 40719..40970 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE74_RS27485 (35489) 35489..36671 + 1183 Protein_36 IS21 family transposase -
QUE74_RS27490 (36668) 36668..37422 + 755 Protein_37 IS21-like element ISEc10 family helper ATPase IstB -
QUE74_RS27495 (37487) 37487..37969 + 483 Protein_38 IS21-like element helper ATPase IstB -
QUE74_RS27500 (38037) 38037..38699 + 663 Protein_39 IS3 family transposase -
QUE74_RS27505 (38757) 38757..39964 - 1208 Protein_40 IS256 family transposase -
QUE74_RS27510 (40448) 40448..40729 - 282 WP_000220564.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
QUE74_RS27515 (40719) 40719..40970 - 252 WP_000121742.1 hypothetical protein Antitoxin
QUE74_RS27520 (41246) 41246..42265 - 1020 WP_024262278.1 IS110 family transposase -
QUE74_RS27525 (43642) 43642..43643 - 2 WP_097638450.1 IS3 family transposase -
QUE74_RS27530 (43975) 43975..44778 - 804 Protein_45 IS256-like element IS1414 family transposase -
QUE74_RS27535 (44950) 44950..45570 - 621 WP_000269722.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - hlyD / hlyD / hlyD / hlyB / hlyB / hlyA / hlyA / hlyC / estIa / faeJ / faeI / faeH / faeF / faeE / faeD / faeC / espP 1..224339 224339
- inside IScluster/Tn - - 33495..69706 36211


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11009.78 Da        Isoelectric Point: 10.5938

>T40727 WP_000220564.1 NZ_AP025742:c40729-40448 [Escherichia sp. 10290]
MTYELAFDRRALKEWQKLGHTIREQFKKKLSERLENPRAPAARLHGHADRYKIKLRSSGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T40727 NZ_AP025742:c40729-40448 [Escherichia sp. 10290]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGTCAGAACGGCTGGAAAATCCACGCGCACCTGCAGCCCGATTACATGGTCATGCTGATCGTTATAAAATCA
AACTTCGTTCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGAGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9181.47 Da        Isoelectric Point: 4.2958

>AT40727 WP_000121742.1 NZ_AP025742:c40970-40719 [Escherichia sp. 10290]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKKPEIVKVSL
DDL

Download         Length: 252 bp

>AT40727 NZ_AP025742:c40970-40719 [Escherichia sp. 10290]
ATGAGTTATCAAATCCTGACAACCACAGCGGCCAGTATTACTGACCTAAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCAATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAAACCGGAAATTGTGAAGGTTAGCCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A6Q014

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0W3C1

References