Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 5120568..5121065 Replicon chromosome
Accession NZ_AP025741
Organism Escherichia sp. 10290

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QUE74_RS25460 Protein ID WP_001173343.1
Coordinates 5120892..5121065 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QUE74_RS25455 Protein ID WP_000398619.1
Coordinates 5120568..5120864 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE74_RS25435 (5117296) 5117296..5118015 - 720 WP_000611290.1 two-component system response regulator DcuR -
QUE74_RS25440 (5118012) 5118012..5119643 - 1632 WP_001216471.1 sensor histidine kinase -
QUE74_RS25445 (5119824) 5119824..5120054 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
QUE74_RS25450 (5120066) 5120066..5120338 + 273 WP_000405650.1 GNAT family N-acetyltransferase -
QUE74_RS25455 (5120568) 5120568..5120864 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUE74_RS25460 (5120892) 5120892..5121065 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QUE74_RS25465 (5121184) 5121184..5122701 - 1518 WP_000003806.1 lysine--tRNA ligase -
QUE74_RS25470 (5122938) 5122938..5124395 - 1458 WP_000856811.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T40725 WP_001173343.1 NZ_AP025741:5120892-5121065 [Escherichia sp. 10290]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T40725 NZ_AP025741:5120892-5121065 [Escherichia sp. 10290]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGCCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT40725 WP_000398619.1 NZ_AP025741:5120568-5120864 [Escherichia sp. 10290]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT40725 NZ_AP025741:5120568-5120864 [Escherichia sp. 10290]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References