Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3735597..3736251 Replicon chromosome
Accession NZ_AP025741
Organism Escherichia sp. 10290

Toxin (Protein)


Gene name cptA Uniprot ID F0JPE9
Locus tag QUE74_RS18885 Protein ID WP_000244774.1
Coordinates 3735597..3736004 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUE74_RS18890 Protein ID WP_000354046.1
Coordinates 3735985..3736251 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE74_RS18865 (3731554) 3731554..3733287 - 1734 WP_000813234.1 single-stranded-DNA-specific exonuclease RecJ -
QUE74_RS18870 (3733293) 3733293..3734003 - 711 WP_000715223.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUE74_RS18875 (3734028) 3734028..3734924 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QUE74_RS18880 (3735036) 3735036..3735557 + 522 WP_001055872.1 flavodoxin FldB -
QUE74_RS18885 (3735597) 3735597..3736004 - 408 WP_000244774.1 protein YgfX Toxin
QUE74_RS18890 (3735985) 3735985..3736251 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUE74_RS18895 (3736503) 3736503..3737483 + 981 WP_000886106.1 tRNA-modifying protein YgfZ -
QUE74_RS18900 (3737679) 3737679..3738338 - 660 WP_000250283.1 hemolysin III family protein -
QUE74_RS18905 (3738502) 3738502..3738813 - 312 WP_001182962.1 N(4)-acetylcytidine aminohydrolase -
QUE74_RS18910 (3738858) 3738858..3740291 + 1434 WP_001354958.1 6-phospho-beta-glucosidase BglA -
QUE74_RS18915 (3740348) 3740348..3741091 - 744 WP_000150658.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16078.05 Da        Isoelectric Point: 11.2511

>T40721 WP_000244774.1 NZ_AP025741:c3736004-3735597 [Escherichia sp. 10290]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T40721 NZ_AP025741:c3736004-3735597 [Escherichia sp. 10290]
GTGGTCCTGTGGCAATCTGATTTACGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGTTAAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGTTGCGTTTACGTTCTGATAGCGGTAAACGTCA
GCACTTGTGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT40721 WP_000354046.1 NZ_AP025741:c3736251-3735985 [Escherichia sp. 10290]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT40721 NZ_AP025741:c3736251-3735985 [Escherichia sp. 10290]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGTGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L6ZX35

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References