Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 566140..566758 Replicon chromosome
Accession NZ_AP025741
Organism Escherichia sp. 10290

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QUE74_RS02715 Protein ID WP_001291435.1
Coordinates 566140..566358 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QUE74_RS02720 Protein ID WP_000344800.1
Coordinates 566384..566758 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE74_RS02680 (561429) 561429..562001 + 573 WP_000779831.1 YbaY family lipoprotein -
QUE74_RS02685 (562032) 562032..562343 - 312 WP_000409907.1 MGMT family protein -
QUE74_RS02695 (562722) 562722..563075 + 354 WP_000878140.1 DUF1428 domain-containing protein -
QUE74_RS02700 (563117) 563117..564667 - 1551 WP_001355176.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QUE74_RS02705 (564831) 564831..565301 - 471 WP_000136192.1 YlaC family protein -
QUE74_RS02710 (565417) 565417..565968 - 552 WP_000102575.1 maltose O-acetyltransferase -
QUE74_RS02715 (566140) 566140..566358 - 219 WP_001291435.1 HHA domain-containing protein Toxin
QUE74_RS02720 (566384) 566384..566758 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QUE74_RS02725 (567305) 567305..570454 - 3150 WP_001132492.1 efflux RND transporter permease AcrB -
QUE74_RS02730 (570477) 570477..571670 - 1194 WP_001353399.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T40697 WP_001291435.1 NZ_AP025741:c566358-566140 [Escherichia sp. 10290]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T40697 NZ_AP025741:c566358-566140 [Escherichia sp. 10290]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTGCGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACTATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT40697 WP_000344800.1 NZ_AP025741:c566758-566384 [Escherichia sp. 10290]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT40697 NZ_AP025741:c566758-566384 [Escherichia sp. 10290]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGATTGCCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAAAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References