Detailed information of TA system TA040696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 531829..532666
• Replicon: chromosome
• Accession: NZ_AP025741
• Organism: Escherichia sp. 10290

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: V0SED1
• Locus tag: QUE74_RS02545
• Protein ID: WP_000227787.1
• Coordinates: 531829..532371 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: B1LJI1
• Locus tag: QUE74_RS02550
• Protein ID: WP_001353405.1
• Coordinates: 532355..532666 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE74_RS02525 (527359)	527359..528270	-	912	WP_000705835.1	2-dehydropantoate 2-reductase	-
QUE74_RS02530 (528438)	528438..528929	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
QUE74_RS02535 (529057)	529057..530421	-	1365	WP_001000949.1	MFS transporter	-
QUE74_RS02540 (530838)	530838..531773	+	936	Protein_499	tetratricopeptide repeat protein	-
QUE74_RS02545 (531829)	531829..532371	-	543	WP_000227787.1	GNAT family N-acetyltransferase	Toxin
QUE74_RS02550 (532355)	532355..532666	-	312	WP_001353405.1	DUF1778 domain-containing protein	Antitoxin
QUE74_RS02555 (532851)	532851..533741	-	891	WP_000971336.1	heme o synthase	-
QUE74_RS02560 (533753)	533753..534082	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QUE74_RS02565 (534082)	534082..534696	-	615	WP_000179807.1	cytochrome o ubiquinol oxidase subunit III	-
QUE74_RS02570 (534686)	534686..536677	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QUE74_RS02575 (536699)	536699..537646	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-96)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19946.24 Da
• Isoelectric Point: 8.8951

>T40696 WP_000227787.1 NZ_AP025741:c532371-531829 [Escherichia sp. 10290]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T40696 NZ_AP025741:c532371-531829 [Escherichia sp. 10290]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCTGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATTGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAGATTGGTGTTGTCAGATTAGTCATGTTAGGGGTAGCGCG
GAAATATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCGGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAACTTTTATACTCGTCTCGGCTTTGTTCAGCTTTCAGCG
AGGCCCAATGTTTTCGGTGCTGTACCGATGTTTTTAGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11370.09 Da
• Isoelectric Point: 10.4825

>AT40696 WP_001353405.1 NZ_AP025741:c532666-532355 [Escherichia sp. 10290]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT40696 NZ_AP025741:c532666-532355 [Escherichia sp. 10290]
ATGCCTGGAAAAACAGTCGCTCTACCCGATATCGATAAAACGCTGAAAAATGCGCGGGTTGAACTCAAAACCAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCAGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• AlphaFold DB: A0A829GE43

Antitoxin

• AlphaFold DB: A0A829G7G3