Detailed information of TA system TA040685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 14033..14564
• Replicon: plasmid pFB-5
• Accession: NZ_AP025731
• Organism: Sphaerotilus sp. FB-5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: I2ALI9
• Locus tag: NGK70_RS26245
• Protein ID: WP_011114071.1
• Coordinates: 14033..14314 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: I2ALI8
• Locus tag: NGK70_RS26250
• Protein ID: WP_011114070.1
• Coordinates: 14301..14564 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK70_RS26230 (CATMQ487_52110)	10568..10927	-	360	WP_011013260.1	transcriptional regulator	-
NGK70_RS26235 (CATMQ487_52120)	11037..11375	+	339	WP_011013259.1	single-stranded DNA-binding protein	-
NGK70_RS26240 (CATMQ487_52130)	11424..12647	+	1224	WP_011013257.1	plasmid replication initiator TrfA	-
NGK70_RS26245 (CATMQ487_52140)	14033..14314	-	282	WP_011114071.1	type II toxin-antitoxin system YafQ family toxin	Toxin
NGK70_RS26250 (CATMQ487_52150)	14301..14564	-	264	WP_011114070.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
NGK70_RS26255 (CATMQ487_52160)	14737..15165	+	429	WP_011114069.1	antirestriction protein	-
NGK70_RS26260 (CATMQ487_52170)	15210..15428	+	219	WP_012478187.1	hypothetical protein	-
NGK70_RS26265 (CATMQ487_52180)	15440..16648	+	1209	WP_012478186.1	hypothetical protein	-
NGK70_RS26270 (CATMQ487_52190)	16665..16922	+	258	WP_012478185.1	transcriptional repressor KorC	-
NGK70_RS26275 (CATMQ487_52200)	17080..17316	+	237	WP_011255200.1	stable inheritance protein KleA	-
NGK70_RS26280 (CATMQ487_52210)	17526..17852	+	327	WP_012478184.1	KleE stable inheritance protein	-
NGK70_RS26285 (CATMQ487_52220)	17854..18264	+	411	WP_011255198.1	DUF2761 domain-containing protein	-
NGK70_RS26290 (CATMQ487_52230)	18385..18693	+	309	WP_011255197.1	transcriptional regulator KorA	-
NGK70_RS26295 (CATMQ487_52240)	18690..19454	+	765	WP_013397098.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..30838	30838
	-	flank	IS/Tn	-	-	7155..10070	2915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (1-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-83)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10743.19 Da
• Isoelectric Point: 7.4750

>T40685 WP_011114071.1 NZ_AP025731:c14314-14033 [Sphaerotilus sp. FB-5]
MRQIERTGQFKRDYKREAKGQHRATLDADLVPVLVALADDQPLEPRHRDHALTGDWKDHRDCHVKPDLVLIYQKPDADTL
RLVRLGSHSELGL

Nucleotide

• Length: 282 bp

>T40685 NZ_AP025731:c14314-14033 [Sphaerotilus sp. FB-5]
ATGCGGCAGATTGAGCGCACCGGCCAGTTCAAGCGCGACTACAAGCGCGAGGCGAAAGGGCAGCACCGGGCCACGCTCGA
CGCCGACCTGGTGCCCGTCCTGGTCGCGCTCGCCGACGACCAGCCGCTGGAACCTCGGCACCGCGACCACGCGCTGACCG
GGGACTGGAAGGATCACCGGGATTGCCACGTCAAGCCCGACCTGGTGCTGATCTACCAGAAGCCCGACGCCGACACGCTG
CGCCTGGTGCGCCTCGGCTCTCACTCCGAACTCGGCTTGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9255.82 Da
• Isoelectric Point: 5.7905

>AT40685 WP_011114070.1 NZ_AP025731:c14564-14301 [Sphaerotilus sp. FB-5]
MAANQLVQTRIDGAIKEEAAAVLAAMGLTVSDAVRLLLTKVAQDKALPFEPLIPNATTIEAMKEARKGKLPRFATVNDLM
ADLHAAD

Nucleotide

• Length: 264 bp

>AT40685 NZ_AP025731:c14564-14301 [Sphaerotilus sp. FB-5]
ATGGCTGCAAACCAGCTCGTACAAACGCGCATCGACGGCGCGATCAAGGAAGAAGCGGCGGCCGTCCTGGCCGCGATGGG
CCTTACCGTGTCCGACGCGGTGCGGCTGCTGCTCACGAAGGTGGCCCAGGACAAGGCGCTGCCCTTCGAGCCGCTGATTC
CGAACGCCACCACCATCGAGGCGATGAAGGAAGCCCGCAAGGGCAAGCTGCCGCGCTTCGCCACCGTCAACGATCTGATG
GCCGATCTGCATGCGGCAGATTGA

Structures

Toxin

• AlphaFold DB: A0A1U9V4I5

Antitoxin

• AlphaFold DB: A0A1U9V3I2