Detailed information of TA system TA040676

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2355129..2355730
• Replicon: chromosome
• Accession: NZ_AP025730
• Organism: Sphaerotilus sp. FB-5

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: NGK70_RS10285
• Protein ID: WP_251973135.1
• Coordinates: 2355545..2355730 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: NGK70_RS10280
• Protein ID: WP_251973134.1
• Coordinates: 2355129..2355521 (-)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK70_RS10250 (CATMQ487_20340)	2350886..2351560	-	675	WP_251973131.1	pilus assembly protein PilX	-
NGK70_RS10255 (CATMQ487_20350)	2351569..2352783	-	1215	WP_251973132.1	PilW family protein	-
NGK70_RS10260	2352938..2353066	-	129	Protein_2030	flagellar biosynthesis protein	-
NGK70_RS10265 (CATMQ487_20370)	2353270..2353935	-	666	WP_251973133.1	GspH/FimT family pseudopilin	-
NGK70_RS10270 (CATMQ487_20380)	2353929..2354432	-	504	WP_256490796.1	type IV pilin protein	-
NGK70_RS10280 (CATMQ487_20390)	2355129..2355521	-	393	WP_251973134.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
NGK70_RS10285 (CATMQ487_20400)	2355545..2355730	-	186	WP_251973135.1	type II toxin-antitoxin system HicA family toxin	Toxin
NGK70_RS10290 (CATMQ487_20410)	2355898..2359347	-	3450	WP_251973136.1	error-prone DNA polymerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-125)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6914.94 Da
• Isoelectric Point: 10.9370

>T40676 WP_251973135.1 NZ_AP025730:c2355730-2355545 [Sphaerotilus sp. FB-5]
VNSKQVIKQLEADGWFLARVKGSHHQFKHPSKPGLVTVKHPDGDIPTPTLYSIQRQAGWRK

Nucleotide

• Length: 186 bp

>T40676 NZ_AP025730:c2355730-2355545 [Sphaerotilus sp. FB-5]
GTGAACAGCAAGCAGGTCATCAAGCAACTGGAAGCCGACGGCTGGTTCCTGGCGCGGGTCAAGGGCTCGCACCACCAGTT
CAAGCACCCCAGCAAGCCGGGGTTGGTGACGGTGAAGCATCCGGATGGCGACATCCCGACGCCCACGCTGTACAGCATCC
AGCGCCAGGCCGGCTGGCGCAAGTGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 14056.82 Da
• Isoelectric Point: 4.6042

>AT40676 WP_251973134.1 NZ_AP025730:c2355521-2355129 [Sphaerotilus sp. FB-5]
MKFTVVLHTDDGTRYGVIVPDLPGCFSGGDTLDAALESAQEAIDLHAETLIEDGADLPERRPIAEHQANPDYAGGVWAVV
EVPVEKYFGPAEKINITLPRVLLARIDDYARAHGASRSGFLAQAARQAMR

Nucleotide

• Length: 393 bp

>AT40676 NZ_AP025730:c2355521-2355129 [Sphaerotilus sp. FB-5]
ATGAAATTCACCGTGGTCCTGCACACCGACGATGGCACGCGCTACGGCGTGATCGTGCCCGACCTGCCCGGCTGCTTCTC
AGGCGGGGACACGCTCGACGCCGCGCTGGAAAGCGCGCAGGAGGCGATCGACCTGCACGCCGAGACCCTGATCGAAGACG
GCGCTGACCTGCCCGAACGCCGGCCCATTGCCGAGCACCAGGCCAATCCGGACTATGCGGGGGGCGTCTGGGCGGTGGTG
GAGGTGCCGGTGGAGAAGTACTTCGGCCCGGCGGAGAAAATCAACATCACCCTACCGCGCGTGCTGCTGGCACGCATCGA
CGACTACGCCCGGGCGCACGGCGCCTCGCGCAGCGGCTTCCTAGCCCAGGCGGCACGGCAAGCCATGCGCTGA