Detailed information of TA system TA040673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1282187..1282851
• Replicon: chromosome
• Accession: NZ_AP025730
• Organism: Sphaerotilus sp. FB-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: NGK70_RS05610
• Protein ID: WP_251972294.1
• Coordinates: 1282429..1282851 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: NGK70_RS05605
• Protein ID: WP_251972293.1
• Coordinates: 1282187..1282429 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK70_RS05585	1279057..1279236	-	180	WP_251972290.1	hypothetical protein	-
NGK70_RS05590 (CATMQ487_11150)	1279359..1280372	-	1014	WP_251969112.1	transposase	-
NGK70_RS05595 (CATMQ487_11160)	1280482..1280703	+	222	WP_251972291.1	hypothetical protein	-
NGK70_RS05600 (CATMQ487_11170)	1280883..1281311	+	429	WP_251972292.1	hypothetical protein	-
NGK70_RS05605 (CATMQ487_11180)	1282187..1282429	+	243	WP_251972293.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
NGK70_RS05610 (CATMQ487_11190)	1282429..1282851	+	423	WP_251972294.1	type II toxin-antitoxin system VapC family toxin	Toxin
NGK70_RS05615 (CATMQ487_11200)	1283139..1283486	+	348	WP_251972295.1	hypothetical protein	-
NGK70_RS05620 (CATMQ487_11210)	1283521..1284036	+	516	WP_251972296.1	hypothetical protein	-
NGK70_RS05625 (CATMQ487_11220)	1284174..1284680	+	507	WP_251972297.1	hypothetical protein	-
NGK70_RS05630 (CATMQ487_11230)	1284658..1285086	-	429	WP_251972298.1	PIN domain nuclease	-
NGK70_RS05635 (CATMQ487_11240)	1285083..1285283	-	201	WP_251972299.1	type II toxin-antitoxin system VapB family antitoxin	-
NGK70_RS05640 (CATMQ487_11250)	1285394..1285954	-	561	WP_251972300.1	histidine phosphatase family protein	-
NGK70_RS05645 (CATMQ487_11260)	1286178..1286510	+	333	WP_251972301.1	cupin domain-containing protein	-
NGK70_RS05650 (CATMQ487_11270)	1286559..1287440	+	882	WP_251972302.1	alpha/beta hydrolase	-
NGK70_RS05655 (CATMQ487_11280)	1287469..1287633	+	165	WP_251972303.1	DUF5668 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (11-131)

Antitoxin

• SpoVT-AbrB domain (8-54)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15263.45 Da
• Isoelectric Point: 7.3596

>T40673 WP_251972294.1 NZ_AP025730:1282429-1282851 [Sphaerotilus sp. FB-5]
MARTPEADRWMLDTNTASFIIRGASPGLKQRLRAIPLSDLRISAVTEGELLYGLARKPQATALKAAVHAFLQHVETLSWD
SAAAQHYATLRARQEAAGRPLGGMDTMIAAHALAQNCILVTNDQAFRRVDGLVVEDWLDG

Nucleotide

• Length: 423 bp

>T40673 NZ_AP025730:1282429-1282851 [Sphaerotilus sp. FB-5]
ATGGCCCGCACCCCTGAGGCCGACCGGTGGATGTTGGACACGAACACGGCCAGCTTCATCATCCGCGGCGCCAGCCCCGG
CCTGAAGCAGAGGTTGCGCGCGATCCCGCTCAGTGACCTGCGGATCTCCGCGGTGACGGAAGGCGAGTTGCTCTACGGCC
TGGCGCGCAAACCGCAGGCCACGGCGCTGAAGGCTGCCGTGCATGCCTTCCTGCAGCACGTCGAGACGCTGTCCTGGGAC
AGCGCCGCCGCCCAGCACTACGCGACCTTGCGTGCCAGGCAGGAGGCGGCCGGCCGGCCGCTCGGCGGCATGGACACGAT
GATCGCGGCGCATGCCCTGGCGCAGAACTGCATCCTGGTGACCAACGATCAGGCTTTCCGGCGGGTGGACGGGCTGGTGG
TGGAGGATTGGCTGGACGGCTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9317.43 Da
• Isoelectric Point: 5.0790

>AT40673 WP_251972293.1 NZ_AP025730:1282187-1282429 [Sphaerotilus sp. FB-5]
MTQTAKVFQNGRSQAVRLPAEFRFDTKEVFVRRDPITGDVILSRRPADWEGFFQLVEQGGIPQDYLTDRRDEPAQSRDLF

Nucleotide

• Length: 243 bp

>AT40673 NZ_AP025730:1282187-1282429 [Sphaerotilus sp. FB-5]
ATGACCCAGACCGCCAAAGTCTTCCAAAACGGCCGCAGCCAGGCCGTTCGGCTGCCGGCCGAGTTCCGCTTCGACACCAA
GGAGGTGTTCGTGCGGCGCGACCCCATCACGGGTGACGTGATCCTCTCCAGGCGTCCGGCAGACTGGGAGGGCTTCTTCC
AGCTGGTGGAGCAGGGCGGTATCCCGCAGGACTACCTGACCGACCGCCGCGATGAGCCGGCGCAGAGCCGGGATCTGTTC
TGA