Detailed information of TA system TA040672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 268033..268622
• Replicon: chromosome
• Accession: NZ_AP025730
• Organism: Sphaerotilus sp. FB-5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: NGK70_RS01230
• Protein ID: WP_251971568.1
• Coordinates: 268239..268622 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: NGK70_RS01225
• Protein ID: WP_251971567.1
• Coordinates: 268033..268242 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NGK70_RS01210 (CATMQ487_02380)	264459..265643	+	1185	WP_251971564.1	iron-containing alcohol dehydrogenase	-
NGK70_RS01215 (CATMQ487_02390)	265690..266142	+	453	WP_251971565.1	thioesterase family protein	-
NGK70_RS01220 (CATMQ487_02400)	266154..267950	+	1797	WP_251971566.1	DUF2235 domain-containing protein	-
NGK70_RS01225 (CATMQ487_02410)	268033..268242	+	210	WP_251971567.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
NGK70_RS01230 (CATMQ487_02420)	268239..268622	+	384	WP_251971568.1	type II toxin-antitoxin system VapC family toxin	Toxin
NGK70_RS01235 (CATMQ487_02430)	268645..270342	-	1698	WP_251971569.1	hypothetical protein	-
NGK70_RS01240 (CATMQ487_02440)	270421..272214	-	1794	WP_251971570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-114)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-43)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13701.00 Da
• Isoelectric Point: 6.9571

>T40672 WP_251971568.1 NZ_AP025730:268239-268622 [Sphaerotilus sp. FB-5]
MTGVLVDTSVWVAHFRQALPALQALLATDQVLCHPLVVLELACGTPPAPRDRSLAHLRSLERAAVATIDETLAMVERERL
YDAGCGAVDVALLASARLTAGARLWTLDKKLHALALRLQVAYADTLH

Nucleotide

• Length: 384 bp

>T40672 NZ_AP025730:268239-268622 [Sphaerotilus sp. FB-5]
ATGACCGGGGTGTTGGTCGACACCTCCGTCTGGGTGGCCCACTTCCGGCAGGCGTTGCCTGCCCTGCAGGCCTTGCTGGC
CACGGATCAAGTGCTCTGCCATCCGTTGGTCGTGCTGGAACTGGCTTGCGGAACGCCTCCCGCACCGCGGGATCGCTCGC
TCGCCCACCTGCGTTCGTTGGAGCGAGCAGCGGTGGCCACCATCGACGAAACCCTGGCCATGGTGGAGCGGGAGCGGCTC
TACGACGCTGGCTGTGGCGCAGTGGACGTCGCCTTGCTTGCGTCGGCGCGCTTGACGGCGGGGGCACGGCTCTGGACCCT
CGACAAGAAGCTGCACGCCCTGGCACTGCGGCTGCAAGTCGCCTACGCAGACACGCTGCACTGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7580.59 Da
• Isoelectric Point: 4.7247

>AT40672 WP_251971567.1 NZ_AP025730:268033-268242 [Sphaerotilus sp. FB-5]
MRTTVTIDDDLYDRALELADPGTEKADLIREAMRTFVRVQAGKRLAALGGAAPEMAEVPRRQPEPSPQS

Nucleotide

• Length: 210 bp

>AT40672 NZ_AP025730:268033-268242 [Sphaerotilus sp. FB-5]
ATGCGCACCACGGTCACCATTGACGACGATCTCTACGACAGGGCCCTGGAGCTGGCCGACCCGGGCACGGAAAAGGCGGA
CCTCATCCGGGAAGCGATGAGGACCTTCGTGCGAGTACAGGCCGGCAAGCGCTTGGCGGCGTTGGGCGGAGCCGCGCCGG
AGATGGCCGAGGTGCCTCGCCGCCAGCCTGAGCCATCCCCTCAGTCATGA