Detailed information of TA system TA040662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2193004..2193533
• Replicon: chromosome
• Accession: NZ_AP025693
• Organism: Staphylococcus aureus strain JICS127

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: M2038_RS10980
• Protein ID: WP_000621175.1
• Coordinates: 2193004..2193366 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: M2038_RS10985
• Protein ID: WP_000948331.1
• Coordinates: 2193363..2193533 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M2038_RS10960 (2189983)	2189983..2190753	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
M2038_RS10965 (2190728)	2190728..2191207	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
M2038_RS10970 (2191209)	2191209..2191535	-	327	WP_001052491.1	anti-sigma factor antagonist	-
M2038_RS10975 (2191654)	2191654..2192655	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
M2038_RS10980 (2193004)	2193004..2193366	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
M2038_RS10985 (2193363)	2193363..2193533	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
M2038_RS10990 (2193618)	2193618..2194766	-	1149	WP_001281145.1	alanine racemase	-
M2038_RS10995 (2194832)	2194832..2195191	-	360	WP_000581200.1	holo-ACP synthase	-
M2038_RS11000 (2195195)	2195195..2195686	-	492	WP_001205910.1	PH domain-containing protein	-
M2038_RS11005 (2195673)	2195673..2197256	-	1584	WP_001294626.1	PH domain-containing protein	-
M2038_RS11010 (2197249)	2197249..2197728	-	480	WP_001287088.1	hypothetical protein	-
M2038_RS11015 (2197936)	2197936..2198496	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T40662 WP_000621175.1 NZ_AP025693:c2193366-2193004 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T40662 NZ_AP025693:c2193366-2193004 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT40662 WP_000948331.1 NZ_AP025693:c2193533-2193363 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT40662 NZ_AP025693:c2193533-2193363 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1