Detailed information of TA system TA040652

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazF-pemI/MazF(toxin)
• Location: 3617778..3618362
• Replicon: chromosome
• Accession: NZ_AP025689
• Organism: Lysinibacillus sp. PLM2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0A3J4J7
• Locus tag: MTP04_RS17960
• Protein ID: WP_036172876.1
• Coordinates: 3617778..3618128 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: MTP04_RS17965
• Protein ID: WP_248574041.1
• Coordinates: 3618132..3618362 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MTP04_RS17935 (MTP04_35280)	3614522..3615298	-	777	WP_248571976.1	RNA polymerase sigma factor SigB	-
MTP04_RS17940 (MTP04_35290)	3615264..3615746	-	483	WP_248571977.1	anti-sigma B factor RsbW	-
MTP04_RS17945 (MTP04_35300)	3615751..3616074	-	324	WP_248571978.1	STAS domain-containing protein	-
MTP04_RS17950 (MTP04_35310)	3616141..3617139	-	999	WP_248571979.1	PP2C family protein-serine/threonine phosphatase	-
MTP04_RS17955 (MTP04_35320)	3617146..3617553	-	408	WP_036172877.1	anti-sigma regulatory factor	-
MTP04_RS17960 (MTP04_35330)	3617778..3618128	-	351	WP_036172876.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
MTP04_RS17965 (MTP04_35340)	3618132..3618362	-	231	WP_248574041.1	transcriptional regulator	Antitoxin
MTP04_RS17970 (MTP04_35350)	3618563..3619675	-	1113	WP_248571980.1	alanine racemase	-
MTP04_RS17975 (MTP04_35360)	3619792..3620793	-	1002	WP_248571981.1	outer membrane lipoprotein carrier protein LolA	-
MTP04_RS17980 (MTP04_35370)	3620900..3621250	-	351	WP_248571982.1	holo-ACP synthase	-
MTP04_RS17985 (MTP04_35380)	3621316..3621924	+	609	WP_036172863.1	rhomboid family intramembrane serine protease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12983.08 Da
• Isoelectric Point: 5.1957

>T40652 WP_036172876.1 NZ_AP025689:c3618128-3617778 [Lysinibacillus sp. PLM2]
MIVKRGDVFFADLSPVVGSEQGGTRPVLIIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAKKYGFERNSVILLEQL
RTIDKSRLTDRITQLDEELMDKVDEALEISLGLMKF

Nucleotide

• Length: 351 bp

>T40652 NZ_AP025689:c3618128-3617778 [Lysinibacillus sp. PLM2]
TTGATTGTGAAACGAGGGGACGTTTTTTTTGCAGATCTATCACCAGTGGTAGGTTCTGAACAAGGTGGAACAAGACCCGT
ATTAATCATTCAAAATGATATAGGAAATCGATTCAGTCCTACAGTAATTATCGCTGCGATTACTGCGCAAATTCAAAAAG
CAAAATTACCAACCCATGTTGAAATAGATGCCAAAAAGTATGGGTTTGAACGAAATTCGGTAATTTTGCTTGAACAATTG
CGTACAATTGATAAATCCAGACTAACCGACCGGATTACTCAATTAGATGAAGAATTAATGGATAAAGTGGACGAAGCCTT
AGAAATAAGTTTAGGATTAATGAAATTTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8797.09 Da
• Isoelectric Point: 6.2156

>AT40652 WP_248574041.1 NZ_AP025689:c3618362-3618132 [Lysinibacillus sp. PLM2]
MLIEFNQTLEQKSARKEEYVIVATRRLSTHKQPNQIREALMKGYVEMSQINLSICSECLHAEYEAEHMVERLVSGG

Nucleotide

• Length: 231 bp

>AT40652 NZ_AP025689:c3618362-3618132 [Lysinibacillus sp. PLM2]
ATGTTAATAGAGTTTAATCAAACACTTGAGCAAAAATCTGCGCGCAAAGAAGAGTATGTAATCGTTGCGACACGTAGATT
ATCAACGCACAAGCAACCAAATCAAATACGGGAAGCTTTAATGAAAGGCTATGTGGAAATGTCACAAATTAATTTGTCAA
TTTGTAGTGAATGTCTACATGCGGAGTATGAAGCGGAGCATATGGTGGAGCGTCTCGTAAGCGGGGGATGA

Structures

Toxin

• AlphaFold DB: A0A0A3J4J7