Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4440054..4440551 Replicon chromosome
Accession NZ_AP025682
Organism Escherichia coli strain Rb-50

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EcoliRb_RS21325 Protein ID WP_001173343.1
Coordinates 4440054..4440227 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag EcoliRb_RS21330 Protein ID WP_001312331.1
Coordinates 4440255..4440551 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EcoliRb_RS21315 (4436724) 4436724..4438181 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
EcoliRb_RS21320 (4438418) 4438418..4439935 + 1518 WP_244331612.1 lysine--tRNA ligase -
EcoliRb_RS21325 (4440054) 4440054..4440227 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EcoliRb_RS21330 (4440255) 4440255..4440551 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EcoliRb_RS21335 (4440779) 4440779..4441051 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
EcoliRb_RS21340 (4441063) 4441063..4441293 - 231 Protein_4185 4Fe-4S mono-cluster protein YjdI -
EcoliRb_RS21345 (4441474) 4441474..4443105 + 1632 WP_001216466.1 sensor histidine kinase -
EcoliRb_RS21350 (4443102) 4443102..4443821 + 720 WP_000611291.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - afaE-VIII / afaD-VIII / afaC-VIII / afaB-VIII / afaA-VIII / afaF-VII 4389219..4440551 51332


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T40626 WP_001173343.1 NZ_AP025682:c4440227-4440054 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T40626 NZ_AP025682:c4440227-4440054 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT40626 WP_001312331.1 NZ_AP025682:c4440551-4440255 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT40626 NZ_AP025682:c4440551-4440255 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References