Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3527930..3528548 Replicon chromosome
Accession NZ_AP025682
Organism Escherichia coli strain Rb-50

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EcoliRb_RS17060 Protein ID WP_001291435.1
Coordinates 3528330..3528548 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag EcoliRb_RS17055 Protein ID WP_000344800.1
Coordinates 3527930..3528304 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EcoliRb_RS17045 (3523020) 3523020..3524213 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
EcoliRb_RS17050 (3524236) 3524236..3527385 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
EcoliRb_RS17055 (3527930) 3527930..3528304 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
EcoliRb_RS17060 (3528330) 3528330..3528548 + 219 WP_001291435.1 HHA domain-containing protein Toxin
EcoliRb_RS17065 (3528721) 3528721..3529272 + 552 WP_137500853.1 maltose O-acetyltransferase -
EcoliRb_RS17070 (3529388) 3529388..3529858 + 471 WP_000136192.1 YlaC family protein -
EcoliRb_RS17075 (3530022) 3530022..3531572 + 1551 WP_137500852.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EcoliRb_RS17080 (3531614) 3531614..3531967 - 354 WP_000878140.1 DUF1428 domain-containing protein -
EcoliRb_RS17090 (3532346) 3532346..3532657 + 312 WP_000409911.1 MGMT family protein -
EcoliRb_RS17095 (3532688) 3532688..3533260 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T40620 WP_001291435.1 NZ_AP025682:3528330-3528548 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T40620 NZ_AP025682:3528330-3528548 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT40620 WP_000344800.1 NZ_AP025682:3527930-3528304 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT40620 NZ_AP025682:3527930-3528304 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References