Detailed information of TA system TA040619

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2871391..2872175
• Replicon: chromosome
• Accession: NZ_AP025682
• Organism: Escherichia coli strain Rb-50

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: EcoliRb_RS13845
• Protein ID: WP_000613626.1
• Coordinates: 2871681..2872175 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: EcoliRb_RS13840
• Protein ID: WP_001110447.1
• Coordinates: 2871391..2871684 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EcoliRb_RS13830 (2866541)	2866541..2867500	-	960	WP_000846329.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
EcoliRb_RS13835 (2868073)	2868073..2871258	+	3186	WP_137501321.1	ribonuclease E	-
EcoliRb_RS13840 (2871391)	2871391..2871684	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
EcoliRb_RS13845 (2871681)	2871681..2872175	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
EcoliRb_RS13850 (2872304)	2872304..2872762	-	459	WP_000526113.1	IS200/IS605-like element IS200C family transposase	-
EcoliRb_RS13855 (2872903)	2872903..2874249	-	1347	WP_000483766.1	IS4-like element IS4 family transposase	-
EcoliRb_RS13860 (2874418)	2874418..2875371	-	954	WP_001212764.1	flagellar hook-associated protein FlgL	-
EcoliRb_RS13865 (2875383)	2875383..2877026	-	1644	WP_137501259.1	flagellar hook-associated protein FlgK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T40619 WP_000613626.1 NZ_AP025682:2871681-2872175 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T40619 NZ_AP025682:2871681-2872175 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGGGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GAGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT40619 WP_001110447.1 NZ_AP025682:2871391-2871684 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT40619 NZ_AP025682:2871391-2871684 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCTGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6