Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4024892..4025546 Replicon chromosome
Accession NZ_AP025677
Organism Escherichia coli strain Rb-23

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag MWM20_RS19470 Protein ID WP_000244765.1
Coordinates 4024892..4025299 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag MWM20_RS19475 Protein ID WP_000354050.1
Coordinates 4025280..4025546 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM20_RS19450 (4020849) 4020849..4022582 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
MWM20_RS19455 (4022588) 4022588..4023298 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
MWM20_RS19460 (4023323) 4023323..4024219 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
MWM20_RS19465 (4024331) 4024331..4024852 + 522 WP_001055874.1 flavodoxin FldB -
MWM20_RS19470 (4024892) 4024892..4025299 - 408 WP_000244765.1 protein YgfX Toxin
MWM20_RS19475 (4025280) 4025280..4025546 - 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
MWM20_RS19480 (4025789) 4025789..4026769 + 981 WP_000886078.1 tRNA-modifying protein YgfZ -
MWM20_RS19485 (4026846) 4026846..4027505 - 660 WP_000250275.1 hemolysin III family protein -
MWM20_RS19490 (4027669) 4027669..4027980 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
MWM20_RS19495 (4028025) 4028025..4029458 + 1434 WP_001339296.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T40601 WP_000244765.1 NZ_AP025677:c4025299-4024892 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T40601 NZ_AP025677:c4025299-4024892 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT40601 WP_000354050.1 NZ_AP025677:c4025546-4025280 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT40601 NZ_AP025677:c4025546-4025280 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References