Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1447204..1447822 Replicon chromosome
Accession NZ_AP025677
Organism Escherichia coli strain Rb-23

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MWM20_RS06770 Protein ID WP_001291435.1
Coordinates 1447204..1447422 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MWM20_RS06775 Protein ID WP_000344800.1
Coordinates 1447448..1447822 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM20_RS06735 (1442491) 1442491..1443063 + 573 WP_000779831.1 YbaY family lipoprotein -
MWM20_RS06740 (1443094) 1443094..1443405 - 312 WP_000409908.1 MGMT family protein -
MWM20_RS06750 (1443784) 1443784..1444137 + 354 WP_000878135.1 DUF1428 family protein -
MWM20_RS06755 (1444179) 1444179..1445729 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MWM20_RS06760 (1445893) 1445893..1446363 - 471 WP_000136192.1 YlaC family protein -
MWM20_RS06765 (1446479) 1446479..1447030 - 552 WP_000102539.1 maltose O-acetyltransferase -
MWM20_RS06770 (1447204) 1447204..1447422 - 219 WP_001291435.1 HHA domain-containing protein Toxin
MWM20_RS06775 (1447448) 1447448..1447822 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MWM20_RS06780 (1448367) 1448367..1451516 - 3150 WP_001132478.1 efflux RND transporter permease AcrB -
MWM20_RS06785 (1451539) 1451539..1452732 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T40584 WP_001291435.1 NZ_AP025677:c1447422-1447204 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T40584 NZ_AP025677:c1447422-1447204 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT40584 WP_000344800.1 NZ_AP025677:c1447822-1447448 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT40584 NZ_AP025677:c1447822-1447448 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References