Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 1044511..1045061 Replicon chromosome
Accession NZ_AP025677
Organism Escherichia coli strain Rb-23

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag MWM20_RS04890 Protein ID WP_001160966.1
Coordinates 1044747..1045061 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag MWM20_RS04885 Protein ID WP_000125566.1
Coordinates 1044511..1044744 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM20_RS04860 (1039584) 1039584..1039871 + 288 WP_000203744.1 ferredoxin-like protein FixX -
MWM20_RS04865 (1039930) 1039930..1041261 + 1332 WP_001183206.1 MFS transporter -
MWM20_RS04870 (1041369) 1041369..1041899 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
MWM20_RS04875 (1041892) 1041892..1043754 + 1863 WP_000377124.1 glutathione-regulated potassium-efflux system protein KefC -
MWM20_RS04880 (1043946) 1043946..1044425 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
MWM20_RS04885 (1044511) 1044511..1044744 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
MWM20_RS04890 (1044747) 1044747..1045061 + 315 WP_001160966.1 CcdB family protein Toxin
MWM20_RS04895 (1045058) 1045058..1045906 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
MWM20_RS04900 (1045913) 1045913..1046290 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
MWM20_RS04905 (1046293) 1046293..1047114 - 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
MWM20_RS04910 (1047111) 1047111..1048100 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
MWM20_RS04915 (1048100) 1048100..1049386 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T40583 WP_001160966.1 NZ_AP025677:1044747-1045061 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T40583 NZ_AP025677:1044747-1045061 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT40583 WP_000125566.1 NZ_AP025677:1044511-1044744 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT40583 NZ_AP025677:1044511-1044744 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References