Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4528740..4529237 Replicon chromosome
Accession NZ_AP025675
Organism Escherichia coli strain Rb-3

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag MWM15_RS21685 Protein ID WP_001173343.1
Coordinates 4528740..4528913 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID S1NQ59
Locus tag MWM15_RS21690 Protein ID WP_001312331.1
Coordinates 4528941..4529237 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM15_RS21675 (4525410) 4525410..4526867 + 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -
MWM15_RS21680 (4527104) 4527104..4528621 + 1518 WP_000003805.1 lysine--tRNA ligase -
MWM15_RS21685 (4528740) 4528740..4528913 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
MWM15_RS21690 (4528941) 4528941..4529237 - 297 WP_001312331.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
MWM15_RS21695 (4529466) 4529466..4529738 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
MWM15_RS21700 (4529750) 4529750..4529980 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
MWM15_RS21705 (4530161) 4530161..4531792 + 1632 WP_001216466.1 sensor histidine kinase -
MWM15_RS21710 (4531789) 4531789..4532508 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T40572 WP_001173343.1 NZ_AP025675:c4528913-4528740 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T40572 NZ_AP025675:c4528913-4528740 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT40572 WP_001312331.1 NZ_AP025675:c4529237-4528941 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Download         Length: 297 bp

>AT40572 NZ_AP025675:c4529237-4528941 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB S1NQ59

References