Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4058821..4059371 Replicon chromosome
Accession NZ_AP025675
Organism Escherichia coli strain Rb-3

Toxin (Protein)


Gene name ccdB Uniprot ID S1PDG4
Locus tag MWM15_RS19375 Protein ID WP_001160966.1
Coordinates 4058821..4059135 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1NMQ0
Locus tag MWM15_RS19380 Protein ID WP_000125568.1
Coordinates 4059138..4059371 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM15_RS19350 (4054496) 4054496..4055782 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
MWM15_RS19355 (4055782) 4055782..4056771 + 990 WP_000241225.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
MWM15_RS19360 (4056768) 4056768..4057589 + 822 WP_001065363.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
MWM15_RS19365 (4057592) 4057592..4057969 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
MWM15_RS19370 (4057976) 4057976..4058824 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
MWM15_RS19375 (4058821) 4058821..4059135 - 315 WP_001160966.1 CcdB family protein Toxin
MWM15_RS19380 (4059138) 4059138..4059371 - 234 WP_000125568.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
MWM15_RS19385 (4059457) 4059457..4059936 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
MWM15_RS19390 (4060128) 4060128..4061990 - 1863 WP_000377109.1 glutathione-regulated potassium-efflux system protein KefC -
MWM15_RS19395 (4061983) 4061983..4062513 - 531 WP_000600732.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
MWM15_RS19400 (4062621) 4062621..4063952 - 1332 WP_001183198.1 MFS transporter -
MWM15_RS19405 (4064011) 4064011..4064298 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.72 Da        Isoelectric Point: 9.0851

>T40564 WP_001160966.1 NZ_AP025675:c4059135-4058821 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T40564 NZ_AP025675:c4059135-4058821 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8560.55 Da        Isoelectric Point: 5.8062

>AT40564 WP_000125568.1 NZ_AP025675:c4059371-4059138 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENTEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT40564 NZ_AP025675:c4059371-4059138 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACA
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G815

Antitoxin

Source ID Structure
AlphaFold DB A0A829GEF2

References