Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3555718..3556336 Replicon chromosome
Accession NZ_AP025675
Organism Escherichia coli strain Rb-3

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MWM15_RS17020 Protein ID WP_001291435.1
Coordinates 3556118..3556336 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MWM15_RS17015 Protein ID WP_000344800.1
Coordinates 3555718..3556092 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM15_RS17005 (3550808) 3550808..3552001 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MWM15_RS17010 (3552024) 3552024..3555173 + 3150 WP_001132481.1 efflux RND transporter permease AcrB -
MWM15_RS17015 (3555718) 3555718..3556092 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MWM15_RS17020 (3556118) 3556118..3556336 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MWM15_RS17025 (3556510) 3556510..3557061 + 552 WP_000102539.1 maltose O-acetyltransferase -
MWM15_RS17030 (3557177) 3557177..3557647 + 471 WP_000136192.1 YlaC family protein -
MWM15_RS17035 (3557811) 3557811..3559361 + 1551 WP_001298569.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MWM15_RS17040 (3559403) 3559403..3559756 - 354 WP_000878138.1 DUF1428 family protein -
MWM15_RS17050 (3560135) 3560135..3560446 + 312 WP_000409908.1 MGMT family protein -
MWM15_RS17055 (3560477) 3560477..3561049 - 573 WP_000779830.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T40561 WP_001291435.1 NZ_AP025675:3556118-3556336 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T40561 NZ_AP025675:3556118-3556336 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT40561 WP_000344800.1 NZ_AP025675:3555718-3556092 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT40561 NZ_AP025675:3555718-3556092 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References