Detailed information of TA system TA040545

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 873802..874634
• Replicon: chromosome
• Accession: NZ_AP025675
• Organism: Escherichia coli strain Rb-3

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: MWM15_RS04200
• Protein ID: WP_000854753.1
• Coordinates: 873802..874176 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: MWM15_RS04205
• Protein ID: WP_001531802.1
• Coordinates: 874266..874634 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWM15_RS04170 (868917)	868917..870065	-	1149	WP_000905914.1	capsule polysaccharide transporter	-
MWM15_RS04175 (870137)	870137..871120	-	984	WP_001335608.1	KpsF/GutQ family sugar-phosphate isomerase	-
MWM15_RS04180 (871930)	871930..872100	-	171	Protein_824	IS110 family transposase	-
MWM15_RS04185 (872442)	872442..873011	-	570	WP_001290247.1	DUF4942 domain-containing protein	-
MWM15_RS04190 (873108)	873108..873305	-	198	WP_000839281.1	DUF957 domain-containing protein	-
MWM15_RS04195 (873317)	873317..873805	-	489	WP_000777541.1	DUF5983 family protein	-
MWM15_RS04200 (873802)	873802..874176	-	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
MWM15_RS04205 (874266)	874266..874634	-	369	WP_001531802.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
MWM15_RS04210 (874797)	874797..875018	-	222	WP_000692329.1	DUF987 domain-containing protein	-
MWM15_RS04215 (875081)	875081..875557	-	477	WP_001186726.1	RadC family protein	-
MWM15_RS04220 (875573)	875573..876058	-	486	WP_001531954.1	antirestriction protein	-
MWM15_RS04225 (876113)	876113..876934	-	822	WP_001531953.1	DUF932 domain-containing protein	-
MWM15_RS04230 (877113)	877113..877202	-	90	WP_112031555.1	DUF905 family protein	-
MWM15_RS04235 (877345)	877345..877800	-	456	WP_000581502.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	871930..872034	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T40545 WP_000854753.1 NZ_AP025675:c874176-873802 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T40545 NZ_AP025675:c874176-873802 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13422.27 Da
• Isoelectric Point: 6.3161

>AT40545 WP_001531802.1 NZ_AP025675:c874634-874266 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNSLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT40545 NZ_AP025675:c874634-874266 [Escherichia coli]
GTGTCAGACGCACTCTCCGGGACAATGCTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACAGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0