Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 713353..714188 Replicon chromosome
Accession NZ_AP025675
Organism Escherichia coli strain Rb-3

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0J5ZPW7
Locus tag MWM15_RS03445 Protein ID WP_000854722.1
Coordinates 713811..714188 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A0K4A940
Locus tag MWM15_RS03440 Protein ID WP_001285576.1
Coordinates 713353..713721 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM15_RS03420 (711078) 711078..711896 + 819 WP_021520251.1 DUF932 domain-containing protein -
MWM15_RS03425 (711988) 711988..712473 + 486 WP_000206656.1 antirestriction protein -
MWM15_RS03430 (712489) 712489..712965 + 477 WP_001360063.1 RadC family protein -
MWM15_RS03435 (713052) 713052..713273 + 222 WP_000692176.1 DUF987 domain-containing protein -
MWM15_RS03440 (713353) 713353..713721 + 369 WP_001285576.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
MWM15_RS03445 (713811) 713811..714188 + 378 WP_000854722.1 TA system toxin CbtA family protein Toxin
MWM15_RS03450 (714185) 714185..714673 + 489 WP_000761669.1 DUF5983 family protein -
MWM15_RS03455 (714693) 714693..714890 + 198 WP_000445281.1 DUF957 domain-containing protein -
MWM15_RS03460 (714975) 714975..715820 + 846 WP_244337969.1 DUF4942 domain-containing protein -
MWM15_RS03470 (716120) 716120..716626 + 507 WP_000228937.1 G/U mismatch-specific DNA glycosylase -
MWM15_RS03475 (716704) 716704..718545 - 1842 WP_000437371.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14103.15 Da        Isoelectric Point: 8.2904

>T40544 WP_000854722.1 NZ_AP025675:713811-714188 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQILLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T40544 NZ_AP025675:713811-714188 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGGTTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13599.36 Da        Isoelectric Point: 6.3159

>AT40544 WP_001285576.1 NZ_AP025675:713353-713721 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELSPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT40544 NZ_AP025675:713353-713721 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGTGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTCAGCCCCCGCCATCAGCATACCGTGACACTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J5ZPW7

Antitoxin

Source ID Structure
AlphaFold DB A0A0K4A940

References