Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 143698..144341 Replicon plasmid pMTY20902_IncFI
Accession NZ_AP025663
Organism Escherichia coli strain TUM20902

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag M2084_RS24225 Protein ID WP_001034046.1
Coordinates 143925..144341 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag M2084_RS24220 Protein ID WP_001261278.1
Coordinates 143698..143928 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2084_RS24190 (139208) 139208..139513 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
M2084_RS24195 (139515) 139515..139733 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
M2084_RS24200 (140325) 140325..140813 + 489 WP_011254646.1 hypothetical protein -
M2084_RS24205 (140847) 140847..141980 - 1134 WP_000545984.1 DUF3800 domain-containing protein -
M2084_RS24210 (142147) 142147..142920 - 774 WP_000905949.1 hypothetical protein -
M2084_RS24215 (142933) 142933..143433 - 501 WP_000528932.1 HEPN family nuclease -
M2084_RS24220 (143698) 143698..143928 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
M2084_RS24225 (143925) 143925..144341 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
M2084_RS24230 (144386) 144386..148180 - 3795 WP_001144731.1 hypothetical protein -
M2084_RS24235 (148561) 148561..148791 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
M2084_RS24240 (148788) 148788..149204 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..170915 170915


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T40539 WP_001034046.1 NZ_AP025663:143925-144341 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T40539 NZ_AP025663:143925-144341 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT40539 WP_001261278.1 NZ_AP025663:143698-143928 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT40539 NZ_AP025663:143698-143928 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References