Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4435310..4435807 Replicon chromosome
Accession NZ_AP025662
Organism Escherichia coli strain TUM20902

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag M2084_RS21290 Protein ID WP_001173343.1
Coordinates 4435310..4435483 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag M2084_RS21295 Protein ID WP_000398619.1
Coordinates 4435511..4435807 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2084_RS21280 (4431981) 4431981..4433438 + 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -
M2084_RS21285 (4433675) 4433675..4435192 + 1518 WP_000003806.1 lysine--tRNA ligase -
M2084_RS21290 (4435310) 4435310..4435483 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
M2084_RS21295 (4435511) 4435511..4435807 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
M2084_RS21300 (4436036) 4436036..4436308 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
M2084_RS21305 (4436320) 4436320..4436550 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
M2084_RS21310 (4436731) 4436731..4438362 + 1632 WP_001216477.1 sensor histidine kinase -
M2084_RS21315 (4438359) 4438359..4439078 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T40531 WP_001173343.1 NZ_AP025662:c4435483-4435310 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T40531 NZ_AP025662:c4435483-4435310 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT40531 WP_000398619.1 NZ_AP025662:c4435807-4435511 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT40531 NZ_AP025662:c4435807-4435511 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACCGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References