Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3630547..3631165 Replicon chromosome
Accession NZ_AP025662
Organism Escherichia coli strain TUM20902

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag M2084_RS17450 Protein ID WP_001291435.1
Coordinates 3630947..3631165 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag M2084_RS17445 Protein ID WP_000344800.1
Coordinates 3630547..3630921 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2084_RS17435 (3625636) 3625636..3626829 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
M2084_RS17440 (3626852) 3626852..3630001 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
M2084_RS17445 (3630547) 3630547..3630921 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
M2084_RS17450 (3630947) 3630947..3631165 + 219 WP_001291435.1 HHA domain-containing protein Toxin
M2084_RS17455 (3631337) 3631337..3631888 + 552 WP_000102559.1 maltose O-acetyltransferase -
M2084_RS17460 (3632004) 3632004..3632474 + 471 WP_000136192.1 YlaC family protein -
M2084_RS17465 (3632638) 3632638..3634188 + 1551 WP_001389864.1 cyclic-guanylate-specific phosphodiesterase PdeB -
M2084_RS17470 (3634230) 3634230..3634583 - 354 WP_000878140.1 DUF1428 domain-containing protein -
M2084_RS17480 (3634962) 3634962..3635273 + 312 WP_000409911.1 MGMT family protein -
M2084_RS17485 (3635304) 3635304..3635876 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T40527 WP_001291435.1 NZ_AP025662:3630947-3631165 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T40527 NZ_AP025662:3630947-3631165 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT40527 WP_000344800.1 NZ_AP025662:3630547-3630921 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT40527 NZ_AP025662:3630547-3630921 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References