Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2578200..2578838 Replicon chromosome
Accession NZ_AP025662
Organism Escherichia coli strain TUM20902

Toxin (Protein)


Gene name hicA Uniprot ID B7N4J4
Locus tag M2084_RS12280 Protein ID WP_000813797.1
Coordinates 2578662..2578838 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag M2084_RS12275 Protein ID WP_076611057.1
Coordinates 2578200..2578616 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2084_RS12255 (2573352) 2573352..2574293 - 942 WP_001251335.1 ABC transporter permease -
M2084_RS12260 (2574294) 2574294..2575307 - 1014 WP_000220413.1 ABC transporter ATP-binding protein -
M2084_RS12265 (2575325) 2575325..2576470 - 1146 WP_000047447.1 ABC transporter substrate-binding protein -
M2084_RS12270 (2576715) 2576715..2578121 - 1407 WP_000760613.1 PLP-dependent aminotransferase family protein -
M2084_RS12275 (2578200) 2578200..2578616 - 417 WP_076611057.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
M2084_RS12280 (2578662) 2578662..2578838 - 177 WP_000813797.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
M2084_RS12285 (2579060) 2579060..2579290 + 231 WP_000494244.1 YncJ family protein -
M2084_RS12290 (2579382) 2579382..2581343 - 1962 WP_001309488.1 23S rRNA 5-hydroxycytidine C2501 synthase -
M2084_RS12295 (2581416) 2581416..2581952 - 537 WP_000429161.1 DNA-binding transcriptional regulator SutR -
M2084_RS12300 (2582005) 2582005..2583216 + 1212 WP_001323162.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6724.76 Da        Isoelectric Point: 11.5666

>T40524 WP_000813797.1 NZ_AP025662:c2578838-2578662 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T40524 NZ_AP025662:c2578838-2578662 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15251.65 Da        Isoelectric Point: 4.5908

>AT40524 WP_076611057.1 NZ_AP025662:c2578616-2578200 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT40524 NZ_AP025662:c2578616-2578200 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J715

Antitoxin

Source ID Structure

References