Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 942999..943653 Replicon chromosome
Accession NZ_AP025662
Organism Escherichia coli strain TUM20902

Toxin (Protein)


Gene name cptA Uniprot ID B7N7E0
Locus tag M2084_RS04595 Protein ID WP_000244769.1
Coordinates 943246..943653 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag M2084_RS04590 Protein ID WP_000354046.1
Coordinates 942999..943265 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2084_RS04570 (939087) 939087..940520 - 1434 WP_001513393.1 6-phospho-beta-glucosidase BglA -
M2084_RS04575 (940565) 940565..940876 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
M2084_RS04580 (941040) 941040..941699 + 660 WP_000250274.1 hemolysin III family protein -
M2084_RS04585 (941776) 941776..942756 - 981 WP_000886049.1 tRNA-modifying protein YgfZ -
M2084_RS04590 (942999) 942999..943265 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
M2084_RS04595 (943246) 943246..943653 + 408 WP_000244769.1 protein YgfX Toxin
M2084_RS04600 (943693) 943693..944214 - 522 WP_001055874.1 flavodoxin FldB -
M2084_RS04605 (944326) 944326..945222 + 897 WP_000806652.1 site-specific tyrosine recombinase XerD -
M2084_RS04610 (945247) 945247..945957 + 711 WP_000715210.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
M2084_RS04615 (945963) 945963..947696 + 1734 WP_000813190.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16062.03 Da        Isoelectric Point: 11.5554

>T40512 WP_000244769.1 NZ_AP025662:943246-943653 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGK
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T40512 NZ_AP025662:943246-943653 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGAAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGTGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT40512 WP_000354046.1 NZ_AP025662:942999-943265 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT40512 NZ_AP025662:942999-943265 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAGTGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829J3T1

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References