Detailed information of TA system TA040505

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 143868..144511
• Replicon: plasmid pMTY13867_IncFI
• Accession: NZ_AP025658
• Organism: Escherichia coli strain TUM13867

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: C7S9Y5
• Locus tag: MWM17_RS24240
• Protein ID: WP_001034046.1
• Coordinates: 144095..144511 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: V0SR71
• Locus tag: MWM17_RS24235
• Protein ID: WP_001261278.1
• Coordinates: 143868..144098 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWM17_RS24205 (139378)	139378..139683	-	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	-
MWM17_RS24210 (139685)	139685..139903	-	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	-
MWM17_RS24215 (140495)	140495..140983	+	489	WP_011254646.1	hypothetical protein	-
MWM17_RS24220 (141017)	141017..142150	-	1134	WP_000545984.1	DUF3800 domain-containing protein	-
MWM17_RS24225 (142317)	142317..143090	-	774	WP_000905949.1	hypothetical protein	-
MWM17_RS24230 (143103)	143103..143603	-	501	WP_000528932.1	HEPN family nuclease	-
MWM17_RS24235 (143868)	143868..144098	+	231	WP_001261278.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MWM17_RS24240 (144095)	144095..144511	+	417	WP_001034046.1	type II toxin-antitoxin system VapC family toxin	Toxin
MWM17_RS24245 (144556)	144556..148350	-	3795	WP_001144731.1	hypothetical protein	-
MWM17_RS24250 (148731)	148731..148961	+	231	WP_001261286.1	type II toxin-antitoxin system VapB family antitoxin	-
MWM17_RS24255 (148958)	148958..149374	+	417	WP_001034044.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA	1..171085	171085

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-53)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14978.31 Da
• Isoelectric Point: 6.7113

>T40505 WP_001034046.1 NZ_AP025658:144095-144511 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Nucleotide

• Length: 417 bp

>T40505 NZ_AP025658:144095-144511 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8853.07 Da
• Isoelectric Point: 4.6437

>AT40505 WP_001261278.1 NZ_AP025658:143868-144098 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Nucleotide

• Length: 231 bp

>AT40505 NZ_AP025658:143868-144098 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9NXF9

Antitoxin

• AlphaFold DB: V0SR71