Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3630807..3631425 Replicon chromosome
Accession NZ_AP025657
Organism Escherichia coli strain TUM13867

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag MWM17_RS17465 Protein ID WP_001291435.1
Coordinates 3631207..3631425 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag MWM17_RS17460 Protein ID WP_000344800.1
Coordinates 3630807..3631181 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MWM17_RS17450 (3625896) 3625896..3627089 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
MWM17_RS17455 (3627112) 3627112..3630261 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
MWM17_RS17460 (3630807) 3630807..3631181 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
MWM17_RS17465 (3631207) 3631207..3631425 + 219 WP_001291435.1 HHA domain-containing protein Toxin
MWM17_RS17470 (3631597) 3631597..3632148 + 552 WP_000102559.1 maltose O-acetyltransferase -
MWM17_RS17475 (3632264) 3632264..3632734 + 471 WP_000136192.1 YlaC family protein -
MWM17_RS17480 (3632898) 3632898..3634448 + 1551 WP_001389864.1 cyclic-guanylate-specific phosphodiesterase PdeB -
MWM17_RS17485 (3634490) 3634490..3634843 - 354 WP_000878140.1 DUF1428 domain-containing protein -
MWM17_RS17495 (3635222) 3635222..3635533 + 312 WP_000409911.1 MGMT family protein -
MWM17_RS17500 (3635564) 3635564..3636136 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T40493 WP_001291435.1 NZ_AP025657:3631207-3631425 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T40493 NZ_AP025657:3631207-3631425 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT40493 WP_000344800.1 NZ_AP025657:3630807-3631181 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT40493 NZ_AP025657:3630807-3631181 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References