Detailed information of TA system TA040490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 3330961..3331599
• Replicon: chromosome
• Accession: NZ_AP025657
• Organism: Escherichia coli strain TUM13867

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: B7N4J4
• Locus tag: MWM17_RS16095
• Protein ID: WP_000813797.1
• Coordinates: 3330961..3331137 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: MWM17_RS16100
• Protein ID: WP_076611057.1
• Coordinates: 3331183..3331599 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWM17_RS16075 (3326583)	3326583..3327755	-	1173	WP_001236223.1	BenE family transporter YdcO	-
MWM17_RS16080 (3327847)	3327847..3328383	+	537	WP_000429161.1	DNA-binding transcriptional regulator SutR	-
MWM17_RS16085 (3328456)	3328456..3330417	+	1962	WP_001309488.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
MWM17_RS16090 (3330509)	3330509..3330739	-	231	WP_000494244.1	YncJ family protein	-
MWM17_RS16095 (3330961)	3330961..3331137	+	177	WP_000813797.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
MWM17_RS16100 (3331183)	3331183..3331599	+	417	WP_076611057.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
MWM17_RS16105 (3331678)	3331678..3333084	+	1407	WP_000760613.1	PLP-dependent aminotransferase family protein	-
MWM17_RS16110 (3333329)	3333329..3334474	+	1146	WP_000047447.1	ABC transporter substrate-binding protein	-
MWM17_RS16115 (3334492)	3334492..3335505	+	1014	WP_000220413.1	ABC transporter ATP-binding protein	-
MWM17_RS16120 (3335506)	3335506..3336447	+	942	WP_001251335.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6724.76 Da
• Isoelectric Point: 11.5666

>T40490 WP_000813797.1 NZ_AP025657:3330961-3331137 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFQGRRSVMPRHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T40490 NZ_AP025657:3330961-3331137 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15251.65 Da
• Isoelectric Point: 4.5908

>AT40490 WP_076611057.1 NZ_AP025657:3331183-3331599 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLTNNDHFIEVPLSIASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT40490 NZ_AP025657:3331183-3331599 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A829J715