Detailed information of TA system TA040460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/Phd-LOTUS_5_Limkain_b1
• Location: 150085..150758
• Replicon: plasmid pTthSNM7-6c
• Accession: NZ_AP025619
• Organism: Thermus thermophilus strain SNM7-6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q53W55
• Locus tag: MWM16_RS12150
• Protein ID: WP_011229190.1
• Coordinates: 150085..150345 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: MWM16_RS12155
• Protein ID: WP_244345463.1
• Coordinates: 150366..150758 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWM16_RS12130 (TthSNM76_24040)	146466..147635	+	1170	WP_011229191.1	cytochrome P450	-
MWM16_RS12135 (TthSNM76_24050)	147632..148330	+	699	WP_011174499.1	lycopene cyclase domain-containing protein	-
MWM16_RS12140 (TthSNM76_24060)	148335..149012	+	678	WP_244345461.1	lysophospholipid acyltransferase family protein	-
MWM16_RS12145 (TthSNM76_24070)	149009..150031	+	1023	WP_244345462.1	glycosyltransferase	-
MWM16_RS12150 (TthSNM76_24080)	150085..150345	+	261	WP_011229190.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Toxin
MWM16_RS12155 (TthSNM76_24090)	150366..150758	+	393	WP_244345463.1	type II toxin-antitoxin system VapC family toxin	Antitoxin
MWM16_RS12160 (TthSNM76_24100)	150856..152424	+	1569	WP_244345464.1	phytoene desaturase family protein	-
MWM16_RS12165 (TthSNM76_24110)	152421..153419	+	999	WP_011229187.1	type 2 isopentenyl-diphosphate Delta-isomerase	-
MWM16_RS12170 (TthSNM76_24120)	153429..153821	+	393	WP_244345465.1	nucleotidyltransferase domain-containing protein	-
MWM16_RS12175 (TthSNM76_24130)	153852..154799	-	948	WP_024119841.1	DUF523 and DUF1722 domain-containing protein	-
MWM16_RS12180 (TthSNM76_24140)	154796..155584	-	789	WP_244345466.1	tryptophan-rich sensory protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..198886	198886

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-67)

Antitoxin

• PIN domain (2-121)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9894.40 Da
• Isoelectric Point: 8.5953

>T40460 WP_011229190.1 NZ_AP025619:150085-150345 [Thermus thermophilus]
VKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPPKTLPNEEVEALFQRQKTG
FREVDF

Nucleotide

• Length: 261 bp

>T40460 NZ_AP025619:150085-150345 [Thermus thermophilus]
GTGAAGGCCATCTGGCAACTCCAAGAGGCCAAAGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCA
GCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGGTGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAGGCCT
CGGTCCTCGAGGCCCTAAGACCCCCCAAGACCCTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGC
TTCCGGGAGGTGGACTTCTGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 14268.66 Da
• Isoelectric Point: 10.6447

>AT40460 WP_244345463.1 NZ_AP025619:150366-150758 [Thermus thermophilus]
VVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWFSGLKASFSGRILPLDAEVMELWGEL
TGNAMREGKPLSPLDAMLAATALRHGLILATRNTEDFRHVPVPLVNPWEG

Nucleotide

• Length: 393 bp

>AT40460 NZ_AP025619:150366-150758 [Thermus thermophilus]
GTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCGCCACCTTAAAGTGGGGGAGGCGTA
CCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGGCCCGAAGGGCCATCCTCAGCCGAT
GGTTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTGGACGCCGAGGTGATGGAGCTCTGGGGCGAGCTC
ACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGCGGCCACGGCCCTGCGGCACGGCCT
GATCCTGGCCACCCGGAACACCGAGGACTTCCGCCACGTTCCCGTGCCCCTCGTGAACCCCTGGGAAGGGTAA

Structures

Toxin

• AlphaFold DB: Q53W55