Detailed information of TA system TA040459

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 1422318..1422970
• Replicon: chromosome
• Accession: NZ_AP025617
• Organism: Thermus thermophilus strain SNM7-6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MWM16_RS07705
• Protein ID: WP_244344273.1
• Coordinates: 1422578..1422970 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q5SKZ1
• Locus tag: MWM16_RS07700
• Protein ID: WP_011227983.1
• Coordinates: 1422318..1422578 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWM16_RS07685 (TthSNM76_15120)	1418612..1419994	+	1383	WP_244344270.1	FAD-linked oxidase C-terminal domain-containing protein	-
MWM16_RS07690 (TthSNM76_15130)	1419981..1421024	+	1044	WP_244344271.1	FAD-binding protein	-
MWM16_RS07695 (TthSNM76_15140)	1421029..1422285	+	1257	WP_244344272.1	glycolate oxidase subunit GlcF	-
MWM16_RS07700 (TthSNM76_15150)	1422318..1422578	+	261	WP_011227983.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
MWM16_RS07705 (TthSNM76_15160)	1422578..1422970	+	393	WP_244344273.1	type II toxin-antitoxin system VapC family toxin	Toxin
MWM16_RS07710 (TthSNM76_15170)	1422955..1424175	+	1221	WP_244344274.1	glycerate kinase	-
MWM16_RS07715 (TthSNM76_15180)	1424180..1424497	+	318	WP_014509970.1	nucleotidyltransferase domain-containing protein	-
MWM16_RS07720 (TthSNM76_15190)	1424487..1424885	+	399	WP_165738949.1	HEPN domain-containing protein	-
MWM16_RS07725 (TthSNM76_15200)	1424878..1425996	+	1119	WP_244344275.1	radical SAM family heme chaperone HemW	-
MWM16_RS07730 (TthSNM76_15210)	1426026..1426427	+	402	WP_124104869.1	PaaI family thioesterase	-
MWM16_RS07735 (TthSNM76_15220)	1426424..1426813	-	390	WP_011227977.1	NADH-quinone oxidoreductase subunit 15	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-85)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14664.87 Da
• Isoelectric Point: 6.8732

>T40459 WP_244344273.1 NZ_AP025617:1422578-1422970 [Thermus thermophilus]
MVLDTSALLGILFREEGYEELLEQLRRTRPHKVAAPTLAEAGIVLGARLGFERVHLLFVLLTELQAEIVPFTERHAREAI
SAYRRYGRGKHPAGLNFGDCLSYALARVEGEPLLYKGQDFDRTDLAWKPS

Nucleotide

• Length: 393 bp

>T40459 NZ_AP025617:1422578-1422970 [Thermus thermophilus]
ATGGTGCTGGACACCTCCGCCCTCCTGGGCATTCTCTTCCGGGAAGAAGGGTACGAGGAGCTTCTGGAGCAACTCCGGCG
CACCCGCCCCCACAAGGTGGCCGCGCCCACCCTGGCCGAAGCGGGGATCGTCCTGGGCGCCCGGCTGGGCTTTGAGCGGG
TGCATCTTCTCTTTGTACTCCTAACCGAGCTCCAGGCCGAGATCGTGCCCTTCACCGAAAGGCATGCCCGGGAGGCCATC
TCCGCTTACCGGCGCTACGGGCGGGGCAAGCACCCCGCCGGGCTCAACTTTGGGGACTGCCTGAGCTACGCCCTGGCCCG
GGTGGAGGGGGAACCCCTCCTCTACAAGGGCCAGGACTTTGACCGGACGGACCTGGCGTGGAAGCCCTCCTGA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 10086.61 Da
• Isoelectric Point: 5.0285

>AT40459 WP_011227983.1 NZ_AP025617:1422318-1422578 [Thermus thermophilus]
MALTIRNKEVERLAEEVARLAGETKTEAVRKALEMRLRELQRKRSFDRVIRFLEEEVWPQIPPELLGKGLSKEEEEEILG
YGKEGY

Nucleotide

• Length: 261 bp

>AT40459 NZ_AP025617:1422318-1422578 [Thermus thermophilus]
ATGGCCCTCACCATCCGCAACAAGGAGGTGGAGCGCCTGGCCGAGGAGGTGGCCCGCCTCGCCGGAGAAACCAAGACGGA
GGCCGTAAGGAAGGCCCTGGAGATGCGCCTTAGGGAACTCCAGAGGAAGCGGAGCTTTGACCGGGTGATCCGCTTCCTGG
AAGAGGAAGTCTGGCCCCAAATCCCGCCGGAGCTTTTGGGGAAGGGGCTCTCCAAGGAAGAGGAGGAAGAAATCCTGGGG
TACGGCAAGGAGGGGTACTGA

Structures

Antitoxin

• AlphaFold DB: A0A7R7YHV5