Detailed information of TA system TA040457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/Phd-LOTUS_5_Limkain_b1
• Location: 33250..33923
• Replicon: plasmid pTthSNM6-6b
• Accession: NZ_AP025614
• Organism: Thermus thermophilus strain SNM6-6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: SNM_RS10815
• Protein ID: WP_244347289.1
• Coordinates: 33250..33510 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A7R7YJT7
• Locus tag: SNM_RS10820
• Protein ID: WP_011174495.1
• Coordinates: 33531..33923 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SNM_RS10785 (TthSNM66_21180)	29631..30800	+	1170	WP_011229191.1	cytochrome P450	-
SNM_RS12455 (TthSNM66_21190)	30797..31495	+	699	WP_011174499.1	lycopene cyclase domain-containing protein	-
SNM_RS10805 (TthSNM66_21200)	31500..32177	+	678	WP_039457849.1	lysophospholipid acyltransferase family protein	-
SNM_RS10810 (TthSNM66_21210)	32174..33196	+	1023	WP_244345462.1	glycosyltransferase	-
SNM_RS10815 (TthSNM66_21220)	33250..33510	+	261	WP_244347289.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Toxin
SNM_RS10820 (TthSNM66_21230)	33531..33923	+	393	WP_011174495.1	type II toxin-antitoxin system VapC family toxin	Antitoxin
SNM_RS10825 (TthSNM66_21240)	34021..35589	+	1569	WP_244347290.1	phytoene desaturase family protein	-
SNM_RS10830 (TthSNM66_21250)	35586..36584	+	999	WP_244347291.1	type 2 isopentenyl-diphosphate Delta-isomerase	-
SNM_RS10835 (TthSNM66_21260)	36594..36986	+	393	WP_244347292.1	nucleotidyltransferase domain-containing protein	-
SNM_RS10840 (TthSNM66_21270)	37017..37964	-	948	WP_024119841.1	DUF523 and DUF1722 domain-containing protein	-
SNM_RS10845 (TthSNM66_21280)	37961..38749	-	789	WP_024119840.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..213005	213005

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-67)

Antitoxin

• PIN domain (2-121)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9953.47 Da
• Isoelectric Point: 9.8088

>T40457 WP_244347289.1 NZ_AP025614:33250-33510 [Thermus thermophilus]
VKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPRKTLPNEEVEALFQRQKTG
FREVDF

Nucleotide

• Length: 261 bp

>T40457 NZ_AP025614:33250-33510 [Thermus thermophilus]
GTGAAGGCCATCTGGCAACTCCAAGAGGCCAAAGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCA
GCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGGTGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAGGCCT
CGGTCCTCGAGGCCCTAAGACCCCGCAAGACCCTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGC
TTCCGGGAGGTGGACTTCTGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 14248.67 Da
• Isoelectric Point: 10.6447

>AT40457 WP_011174495.1 NZ_AP025614:33531-33923 [Thermus thermophilus]
VVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAEVMELWGEL
TGNAMREGKPLSPLDAMLAATALRHGLILATRNTEEFRHVPVPLVNPWEG

Nucleotide

• Length: 393 bp

>AT40457 NZ_AP025614:33531-33923 [Thermus thermophilus]
GTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCGCCACCTTAAAGTGGGGGAGGCGTA
CCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGGCCCGAAGGGCCATCCTCAGCCGAT
GGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTGGACGCCGAGGTGATGGAGCTCTGGGGCGAGCTC
ACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGCGGCCACGGCCCTGCGGCACGGCCT
GATCCTGGCCACCCGGAACACCGAGGAGTTCCGCCACGTTCCCGTGCCCCTCGTGAACCCCTGGGAAGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7R7YJT7