Detailed information of TA system TA040453

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/Phd-LOTUS_5_Limkain_b1
• Location: 177041..177714
• Replicon: plasmid pTthSNM3-3b
• Accession: NZ_AP025610
• Organism: Thermus thermophilus strain SNM3-3

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: MWM13_RS11625
• Protein ID: WP_244347289.1
• Coordinates: 177041..177301 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A3P4AVG9
• Locus tag: MWM13_RS11630
• Protein ID: WP_008631493.1
• Coordinates: 177322..177714 (+)
• Length: 131 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MWM13_RS11595 (TthSNM33_22970)	173422..174591	+	1170	WP_011229191.1	cytochrome P450	-
MWM13_RS13065 (TthSNM33_22980)	174588..175286	+	699	WP_011174499.1	lycopene cyclase domain-containing protein	-
MWM13_RS11615 (TthSNM33_22990)	175291..175968	+	678	WP_039457849.1	lysophospholipid acyltransferase family protein	-
MWM13_RS11620 (TthSNM33_23000)	175965..176987	+	1023	WP_011174497.1	glycosyltransferase	-
MWM13_RS11625 (TthSNM33_23010)	177041..177301	+	261	WP_244347289.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Toxin
MWM13_RS11630 (TthSNM33_23020)	177322..177714	+	393	WP_008631493.1	type II toxin-antitoxin system VapC family toxin	Antitoxin
MWM13_RS11635 (TthSNM33_23030)	177812..179380	+	1569	WP_244348116.1	phytoene desaturase family protein	-
MWM13_RS11640 (TthSNM33_23040)	179377..180375	+	999	WP_244348117.1	type 2 isopentenyl-diphosphate Delta-isomerase	-
MWM13_RS11645 (TthSNM33_23050)	180385..180777	+	393	WP_244345465.1	nucleotidyltransferase domain-containing protein	-
MWM13_RS11650 (TthSNM33_23060)	180808..181755	-	948	WP_024119841.1	DUF523 and DUF1722 domain-containing protein	-
MWM13_RS11655 (TthSNM33_23070)	181752..182540	-	789	WP_024119840.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..241732	241732

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (4-67)

Antitoxin

• PIN domain (2-121)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9953.47 Da
• Isoelectric Point: 9.8088

>T40453 WP_244347289.1 NZ_AP025610:177041-177301 [Thermus thermophilus]
VKAIWQLQEAKARFSEVVERALKGEPQLVLRRGKKAVVVLDWETYARLTGEEASVLEALRPRKTLPNEEVEALFQRQKTG
FREVDF

Nucleotide

• Length: 261 bp

>T40453 NZ_AP025610:177041-177301 [Thermus thermophilus]
GTGAAGGCCATCTGGCAACTCCAAGAGGCCAAAGCCCGCTTTTCCGAGGTGGTGGAGCGGGCCCTCAAGGGGGAGCCCCA
GCTCGTCCTCCGGCGGGGTAAGAAGGCGGTGGTGGTGCTGGACTGGGAGACCTACGCCCGGCTCACGGGGGAAGAGGCCT
CGGTCCTCGAGGCCCTAAGACCCCGCAAGACCCTCCCCAATGAGGAGGTGGAAGCCCTCTTCCAAAGGCAAAAGACGGGC
TTCCGGGAGGTGGACTTCTGA

Antitoxin

Protein

• Length: 131 a.a.
• Molecular weight: 14234.65 Da
• Isoelectric Point: 10.6447

>AT40453 WP_008631493.1 NZ_AP025610:177322-177714 [Thermus thermophilus]
VVSEAVKRRPHPKVVAFLRHLKVGEAYLSALTLGELVQGVARAPEARRAILSRWLSGLKASFSGRILPLDAEVMELWGEL
TGNAMREGKPLSPLDAMLAATALRHGLILATRNTEDFRHVPVPLVNPWEG

Nucleotide

• Length: 393 bp

>AT40453 NZ_AP025610:177322-177714 [Thermus thermophilus]
GTGGTCTCCGAGGCCGTCAAGCGGCGGCCTCACCCCAAGGTGGTGGCCTTCCTGCGCCACCTTAAAGTGGGGGAGGCGTA
CCTCTCCGCCCTCACCTTGGGCGAACTGGTCCAGGGGGTGGCCCGGGCCCCGGAGGCCCGAAGGGCCATCCTCAGCCGAT
GGCTCTCGGGGCTGAAGGCGAGCTTCTCGGGGCGGATCCTTCCCCTGGACGCCGAGGTGATGGAGCTCTGGGGCGAGCTC
ACCGGAAACGCCATGAGGGAGGGCAAGCCCCTCTCTCCCCTAGACGCCATGCTGGCGGCCACGGCCCTGCGGCACGGCCT
GATCCTGGCCACCCGGAACACCGAGGACTTCCGCCACGTTCCCGTGCCCCTCGTGAACCCCTGGGAAGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A3P4AVG9